Herniter Ira A, Muñoz-Amatriaín María, Lo Sassoum, Guo Yi-Ning, Close Timothy J
Department of Botany and Plant Sciences, University of California Riverside, Riverside, CA, 92521-0124.
Department of Botany and Plant Sciences, University of California Riverside, Riverside, CA, 92521-0124
G3 (Bethesda). 2018 Oct 3;8(10):3347-3355. doi: 10.1534/g3.118.200521.
Seed coat color is an important part of consumer preferences for cowpea ( [L.] Walp). Color has been studied in numerous crop species and has often been linked to loci controlling the anthocyanin biosynthesis pathway. This study makes use of available resources, including mapping populations, a reference genome, and a high-density single nucleotide polymorphism genotyping platform, to map the black seed coat and purple pod tip color traits, with the gene symbol , in cowpea. Several gene models encoding MYB domain protein 113 were identified as candidate genes. MYB domain proteins have been shown in other species to control expression of genes encoding enzymes for the final steps in the anthocyanin biosynthesis pathway. PCR analysis indicated that a presence/absence variation of one or more MYB113 genes may control the presence or absence of black pigment. A PCR marker has been developed for the MYB113 gene , a candidate gene for black seed coat color in cowpea.
种皮颜色是豇豆（[L.] Walp）消费者偏好的重要组成部分。颜色已在众多作物物种中得到研究，并且常常与控制花青素生物合成途径的基因座相关联。本研究利用现有资源，包括作图群体、参考基因组和高密度单核苷酸多态性基因分型平台，对豇豆中黑种皮和紫荚尖颜色性状（基因符号为 ）进行定位。鉴定出几个编码MYB结构域蛋白113的基因模型作为候选基因。在其他物种中已表明，MYB结构域蛋白可控制花青素生物合成途径最后几步中编码酶的基因的表达。PCR分析表明，一个或多个MYB113基因的存在/缺失变异可能控制黑色色素的有无。已开发出一种针对MYB113基因的PCR标记，该基因是豇豆黑种皮颜色的候选基因。
