Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Department of Grass and ForageScience, College of Animal Science and Technology, China Agricultural University, Beijing, China.
J Sci Food Agric. 2019 Feb;99(3):1384-1396. doi: 10.1002/jsfa.9331. Epub 2018 Oct 25.
Alfalfa (Medicago sativa L.), the primary forage crop throughout the world, is sensitive to salt stress during the germination stage. To investigate the response of alfalfa to salt stress, a comprehensive proteomic analysis was performed comparing alfalfa cultivars that differ in salinity tolerance in the early seedling.
Five cultivars were examined for salt tolerance, and the most salt-tolerant cultivar, ZhongmuNo.3, and the most salt-sensitive cultivar, Daxiyang, were compared in terms of their physiological and proteomic responses. The two alfalfa cultivars seeds were exposed to 0 mmolL or 200 mmolL NaCl salt treatment for 10 days. Salt stress significantly reduced young seedling growth and the cotyledons' chlorophyll content; meanwhile, it increased the cotyledons' H O and malondialdehyde (MDA) levels, all of which were less adversely affected in ZhongmuNo.3 than in Daxiyang. A total of 51 spots (24 and 27 protein spots in the salt-sensitive and salt-tolerant cultivars, respectively) were identified as significantly differentially expressed using two-dimensional electrophoresis analysis. The proteins that were associated with salt tolerance included antioxidants/detoxifying enzymes, molecular chaperones, energy metabolic enzymes, a secondary metabolic enzyme, and pathogenesis-related proteins.
Under salt stress, ZhongmuNo.3 possessed a higher capacity for reactive oxygen species (ROS) scavenging, a more abundant energy supply, and stronger photosynthesis than the salt-sensitive cultivar Daxiyang, and these physiological processes may be the primary contributors to salt tolerance in ZhongmuNo.3. These advanced proteome data expand our knowledge of the physiology of the response of alfalfa to salt stress, providing a potentially valuable foundation for molecular breeding to enhance salt tolerance. © 2018 Society of Chemical Industry.
紫花苜蓿（Medicago sativa L.）是全世界主要的饲料作物，在发芽阶段对盐胁迫敏感。为了研究紫花苜蓿对盐胁迫的响应，采用比较不同耐盐性的苜蓿品种在早期幼苗的综合蛋白质组学分析方法进行了研究。
对 5 个品种进行了耐盐性研究，以耐盐性最强的中苜 3 号和最敏感的大盐阳进行比较，比较它们的生理和蛋白质组响应。将两个紫花苜蓿品种的种子暴露于 0 mmolL 或 200 mmolL NaCl 盐处理 10 天。盐胁迫显著降低了幼苗的生长和子叶的叶绿素含量；同时，它增加了子叶的 H 2 O 2 和丙二醛（MDA）水平，中苜 3 号的这些变化均小于大盐阳。通过二维电泳分析，共鉴定出 51 个差异表达的点（盐敏感和耐盐品种分别为 24 和 27 个蛋白点）。与耐盐性相关的蛋白质包括抗氧化剂/解毒酶、分子伴侣、能量代谢酶、次生代谢酶和与发病机制相关的蛋白质。
在盐胁迫下，中苜 3 号比盐敏感品种大盐阳具有更高的活性氧（ROS）清除能力、更丰富的能量供应和更强的光合作用，这些生理过程可能是中苜 3 号耐盐的主要原因。这些先进的蛋白质组数据扩展了我们对紫花苜蓿响应盐胁迫生理的认识，为提高耐盐性的分子育种提供了有价值的基础。© 2018 化学工业协会。
