Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan.
Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan.
Int J Biol Macromol. 2018 Dec;120(Pt A):952-958. doi: 10.1016/j.ijbiomac.2018.08.112. Epub 2018 Aug 23.
Microelements play pivotal roles for fungal/plant development and end-use properties. In this study, we examined the production and characterization of valuable sulfated polysaccharides (SPSs) with biological benefits from Antrodia cinnamomea and fine-tuning of mycelial culture conditions. Using various sulfated salts (e.g. CuSO, FeSO and ZnSO) to feed A. cinnamomea, we found that CuSO and ZnSO increased 25% and 20% of mycelium yields, respectively. We further isolated the SPSs from CuSO, FeSO and ZnSO-feeding of A. cinnamomea (called CuFSPS, FeFSPS and ZnFSPS, respectively) and found that CuSO and ZnSO significantly promoted SPS production. By contrast, FeSO did not change the yields of mycelium and SPS from A. cinnamomea. Characteristic studies have revealed that these sulfated salts did not significantly induce change in the sulfation and the sugar contents of SPS. However, the galactose and glucose contents in ZnFSPS were increased to the value of 249 and 1038 μmol/g, respectively. In addition, in regard to area percentages, while the major SPSs species were low-molecular-weight SPSs (<23 kDa), the sulfated salts increased the area percentages of molecular size in the range of 200-500 kDa. Anticancer function studies showed that those SPSs inhibit the cell viability 35-45% at 800 μg/ml of lung cancer A549 cells via downregulation of EGFR signaling. Our study is the first to identify the efficacy of microelements in the enhancement of mycelia yield and SPS, in which CuSO and ZnSO enhanced mycelia growth and increased the production of SPS. Our finding suggests that ZnSO may play roles in regulating the SPS assembling. Moreover, those SPSs derived from feeding A. cinnamomea with microelements may be useful as a potential agent for inhibition of lung cancer viability.
微量元素在真菌/植物的发育和终端产品特性中起着关键作用。在这项研究中，我们研究了从密纹薄孔菌中生产和表征具有生物益处的有价值的硫酸化多糖（SPS），并对菌丝体培养条件进行了微调。我们使用各种硫酸盐（例如 CuSO、FeSO 和 ZnSO）来喂养密纹薄孔菌，发现 CuSO 和 ZnSO 分别使菌丝体产量增加了 25%和 20%。我们进一步从 CuSO、FeSO 和 ZnSO 喂养的密纹薄孔菌中分离出 SPS（分别称为 CuFSPS、FeFSPS 和 ZnFSPS），发现 CuSO 和 ZnSO 显著促进了 SPS 的产生。相比之下，FeSO 并没有改变密纹薄孔菌的菌丝体和 SPS 的产量。特征研究表明，这些硫酸盐并没有显著改变 SPS 的硫酸化和糖含量。然而，ZnFSPS 中的半乳糖和葡萄糖含量分别增加到 249 和 1038 μmol/g。此外，在面积百分比方面，虽然主要的 SPS 物种是低分子量的 SPS（<23 kDa），但硫酸盐增加了 200-500 kDa 范围内的分子量的面积百分比。抗癌功能研究表明，这些 SPS 通过下调 EGFR 信号通路，在 800 μg/ml 的肺癌 A549 细胞中抑制细胞活力 35-45%。我们的研究首次确定了微量元素在增强菌丝体产量和 SPS 中的功效，其中 CuSO 和 ZnSO 增强了菌丝体的生长并增加了 SPS 的产量。我们的发现表明，ZnSO 可能在调节 SPS 组装中发挥作用。此外，从密纹薄孔菌用微量元素喂养中得到的 SPS 可能可用作抑制肺癌活力的潜在试剂。
