National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, Taiwan; Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan.
Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan.
Carbohydr Polym. 2018 Dec 15;202:536-544. doi: 10.1016/j.carbpol.2018.09.009. Epub 2018 Sep 6.
Antrodia cinnamomea is a polyporaceous medicinal and native fungus in Taiwan. In this study, we found that AC-SPS-F3, a sulfated glucan from A. cinnamomea, reduced lung cancer cell viability via inhibition of EGFR and mTOR activity. The co-administration of AC-SPS-F3 and cisplatin synergistically inhibited lung cancer cell viability. We identified AC-SPS-F3 was a sulfated β-(1→4)-d-glucan with two long 1,6-branches in each repeat unit. The FT-IR absorption at 1341 cm and 887 cm confirmed the existence of sulfates. The proposed repeat unit of AC-SPS-F3, including the types of main skeleton and side chains, as well as the position of the minor galactopyranosyl and mannopyranosyl residues, were proposed according to the 1D and 2D NMR spectra, shown as follows: The features for the proposed repeat unit of AC-SPS-F3 included two long β-(1→6)-Glcp branches, a very high ratio of sulfate substitution, and partial 2-O and 4-O substituents evenly distributed on the β-(1→6)-Glcp branches. The present study is first to characterize the highly branched sulfated polysaccharides and elucidates its anti-cancer functions.
樟芝是台湾特有的药用多孔菌真菌。在这项研究中，我们发现来自樟芝的一种硫酸化葡聚糖 AC-SPS-F3 通过抑制 EGFR 和 mTOR 活性降低肺癌细胞活力。AC-SPS-F3 与顺铂联合使用可协同抑制肺癌细胞活力。我们确定 AC-SPS-F3 是一种带有两个长 1,6-支链的硫酸化 β-(1→4)-d-葡聚糖。在 1341cm 和 887cm 处的 FT-IR 吸收证实了硫酸盐的存在。根据 1D 和 2D NMR 光谱，提出了 AC-SPS-F3 的重复单元的类型，包括主骨架和侧链的类型，以及少量半乳糖吡喃基和甘露吡喃基残基的位置，如下所示：AC-SPS-F3 的重复单元的特征包括两个长的β-(1→6)-Glcp 支链、非常高的硫酸化取代率以及β-(1→6)-Glcp 支链上均匀分布的部分 2-O 和 4-O 取代基。本研究首次对高度支化的硫酸化多糖进行了表征，并阐明了其抗癌功能。
