National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, Taiwan; Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan.
National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, Taiwan.
Int J Biol Macromol. 2021 Feb 15;170:307-316. doi: 10.1016/j.ijbiomac.2020.12.135. Epub 2020 Dec 29.
Antrodia cinnamomea is a precious Polyporaceous fungus with various bioactivities. This study reports the chemical identification and biological activities of sulfomalonoglucan, a sulfated polysaccharide (SPS), from the sodium sulfate enriched medium of the title fungus. The SPS-containing fraction was separated by gel filtration chromatography (GFC) to give the title SPS (denoted as Na10_SPS-F3). By analyzing the evidence for key inter-glycosidic linkages in the 1D and 2D NMR spectroscopic data, one possible repeat unit was proposed as: Na10_SPS-F3 inhibited the secretion of tumor necrosis factor (TNF-α) and interleukin (IL)-6 after lipopolysaccharide (LPS) stimulation in RAW264.7 macrophages. Mechanistically, Na10_SPS-F3 downregulated TGFRII also attenuated the LPS-induced IκB-α degradation. Moreover, Na10_SPS-F3 inhibited lung cancer cell H1975 EGFR/ERK signaling. This is the first paper reporting a 3-O-sulfomalonyl glucan (Na10_SPS-F3) with eight 1,4-β-Glc moieties connected with ten 1,4-α-Glc moieties from Antrodia cinnamomea and its anti-inflammatory and anti-cancer activities.
樟芝是一种具有多种生物活性的珍贵多孔菌。本研究报道了从标题真菌的亚硫酸钠富集培养基中分离出的一种硫酸化多糖（SPS）——磺基化甘露葡聚糖的化学鉴定和生物活性。含 SPS 的部分通过凝胶过滤色谱（GFC）分离，得到标题 SPS（表示为 Na10_SPS-F3）。通过分析 1D 和 2D NMR 光谱数据中糖苷键的关键证据，提出了一个可能的重复单元：Na10_SPS-F3 抑制脂多糖（LPS）刺激 RAW264.7 巨噬细胞后肿瘤坏死因子（TNF-α）和白细胞介素（IL）-6 的分泌。在机制上，Na10_SPS-F3 下调 TGFRII 也减弱了 LPS 诱导的 IκB-α 降解。此外，Na10_SPS-F3 抑制肺癌细胞 H1975 EGFR/ERK 信号。这是第一篇报道从樟芝中分离出具有八个 1,4-β-Glc 单元与十个 1,4-α-Glc 单元连接的 3-O-磺基化甘露聚糖（Na10_SPS-F3）及其抗炎和抗癌活性的论文。
