State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, PR China; University of Chinese Academy of Sciences, Beijing, PR China.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, PR China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, PR China.
Fish Shellfish Immunol. 2018 Nov;82:400-407. doi: 10.1016/j.fsi.2018.08.044. Epub 2018 Aug 23.
A 50-day feeding trial was carried out to evaluate the partial replacement of fishmeal by yeast culture (YC) on growth performance, immune response and resistance against Aeromonas hydrophila in gibel carp CAS Ⅲ (Carassius auratus gibelio). Four isonitrogenous and isoenergetic practical diets including a basal diet (the control diet containing 10% fish meal, D0) and three yeast culture diets (substituting 20%, 40%, 60% of the fishmeal in the basal diet, D20, D40 and D60, respectively) were formulated. Each diet was randomly allocated to quadruplicate fish groups (average initial body weight: 28.70 ± 0.03 g) reared in a recirculating system. After the growth trial, bacterial challenge test was conducted. The results showed that no noteworthy variations in feed intake, growth performance and morphology indices were found among groups (P > 0.05). YC Supplemented diet exerted little significant influence on plasma parameters including triglyceride, glucose, creatinine, total protein and urea nitrogen compared with the control group (P > 0.05). No obvious variations were found in activities of plasma lysozyme, IgM, MPO and SOD before challenge test among dietary treatments (P > 0.05), whereas considerable higher value of the foresaid indicators was discovered in D40 after bacteria challenge (P < 0.05). Transcriptional levels of Toll like receptor 2 (TLR2), myeloid differentiation factor 88 (MyD88), Toll/IL-1 receptor domain-containing adaptor protein (TIRAP) and interleukin-1β (IL-1β) in spleen after challenge were significantly up-regulated in D40 compared with D0 (P < 0.05). Cumulative survival rate in D40 and D60 were significantly higher than those in D0 and D20 (P < 0.05). Taken together, yeast culture could be a suitable fishmeal alternative in diets of gibel carp and dietary inclusion of 4 g YC per 100 g diet enhanced the immunity and disease resistance of gibel carp partly via TLR2 pathway.
进行了一项为期 50 天的喂养试验，以评估酵母培养物（YC）替代部分鱼粉对吉富罗非鱼生长性能、免疫反应和抗嗜水气单胞菌能力的影响。共配制了四种等氮等能的实用饲料，包括基础饲料（含 10%鱼粉，D0）和三种酵母培养物饲料（分别替代基础饲料中 20%、40%和 60%的鱼粉，D20、D40 和 D60）。将每种饲料随机分配到四个重复的鱼类养殖组（平均初始体重：28.70±0.03g）中，在循环系统中进行养殖。生长试验结束后进行细菌攻毒试验。结果表明，各组间摄食率、生长性能和形态指标无显著差异（P>0.05）。与对照组相比，YC 补充饲料对血浆参数（甘油三酯、葡萄糖、肌酐、总蛋白和尿素氮）影响不大（P>0.05）。攻毒前，各饲料处理组间血浆溶菌酶、IgM、MPO 和 SOD 活性无明显差异（P>0.05），而在细菌攻毒后，D40 组上述指标值显著升高（P<0.05）。攻毒后，D40 组脾脏中 Toll 样受体 2（TLR2）、髓样分化因子 88（MyD88）、Toll/IL-1 受体域包含衔接蛋白（TIRAP）和白细胞介素 1β（IL-1β）的转录水平显著高于 D0 组（P<0.05）。D40 和 D60 的累计存活率显著高于 D0 和 D20（P<0.05）。综上所述，酵母培养物可作为吉富罗非鱼饲料中的一种适宜的鱼粉替代品，饲料中添加 4g YC/100g 饲料可部分通过 TLR2 途径增强吉富罗非鱼的免疫力和抗病能力。
