Department of Infection Control, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, PR China.
Department of Cardiothoracic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, PR China.
J Glob Antimicrob Resist. 2018 Dec;15:256-261. doi: 10.1016/j.jgar.2018.08.013. Epub 2018 Aug 23.
The aim of this study was to investigate the carriage rates of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) among healthcare workers (HCWs) at a tertiary-care hospital in southeastern China and to analyse the epidemiological relatedness of the S. aureus isolates.
A total of 242 pharynx swabs were collected in March-April 2016 from 242 HCWs working in seven different wards and departments. Isolates were identified as S. aureus based on morphology, coagulase test and Vitek test. Antimicrobial susceptibility testing was performed by using the Kirby-Bauer disk diffusion method. The epidemiological relatedness of the S. aureus isolates was determined by pulsed-field gel electrophoresis (PFGE).
From the 242 HCWs, 70 (28.9%) S. aureus strains, including 10 (4.1%) MRSA strains, were identified during screening, with the highest MRSA rate detected in nurses (8/107; 7.5%). Carriage rates of S. aureus and MRSA among surgical HCWs were 30.0% (63/210) and 4.3% (9/210), respectively. Methicillin-susceptible S. aureus (MSSA) isolates were grouped by PFGE analysis into five similar groups (A-E), with most isolates belonging to groups D and E, accounting for 63.0% of isolates. Furthermore, two MRSA isolates from gastrointestinal surgery had identical PFGE patterns.
The prevalence of S. aureus colonisation among HCWs was high in this hospital, although the MRSA carriage rate in surgical wards was low. The identical PFGE pattern detected in two MRSA isolates from the same surgical department supports that effective control for possible cross-infection should be implemented.
本研究旨在调查中国东南部一家三级医院医护人员（HCWs）中金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的携带率，并分析金黄色葡萄球菌分离株的流行病学相关性。
2016 年 3 月至 4 月，从 7 个不同病房和科室的 242 名 HCWs 中采集了 242 份咽拭子。根据形态学、凝固酶试验和 Vitek 试验将分离物鉴定为金黄色葡萄球菌。采用 Kirby-Bauer 纸片扩散法进行抗菌药物敏感性试验。通过脉冲场凝胶电泳（PFGE）确定金黄色葡萄球菌分离株的流行病学相关性。
在 242 名 HCWs 中，70 株（28.9%）金黄色葡萄球菌，包括 10 株（4.1%）耐甲氧西林金黄色葡萄球菌（MRSA），在筛查中被鉴定出来，护士中 MRSA 检出率最高（8/107；7.5%）。外科 HCWs 金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌的携带率分别为 30.0%（63/210）和 4.3%（9/210）。PFGE 分析将耐甲氧西林金黄色葡萄球菌分离株分为 5 个相似组（A-E），其中大多数分离株属于 D 和 E 组，占分离株的 63.0%。此外，来自胃肠外科的 2 株耐甲氧西林金黄色葡萄球菌分离株具有相同的 PFGE 模式。
该医院 HCWs 金黄色葡萄球菌定植率较高，尽管外科病房耐甲氧西林金黄色葡萄球菌携带率较低。同一外科科室的 2 株耐甲氧西林金黄色葡萄球菌分离株检测到相同的 PFGE 模式支持应实施有效的控制措施，防止可能的交叉感染。
