State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Shanghai Waterway Engineering Design and Consulting Co., Ltd., Shanghai 200092, China.
Bioresour Technol. 2018 Nov;268:692-699. doi: 10.1016/j.biortech.2018.08.053. Epub 2018 Aug 16.
Nonylphenol (NP) biodegradation under anaerobic conditions is difficult. Here, enhancement of anaerobic NP biodegradation mainly by regulating the role of acidogenic bacteria during anaerobic fermentation of waste activated sludge (WAS) for short-chain fatty acids production is reported. The maximum degradation efficiency of NP (69.4%) was achieved under conditions of pH 10.0 and 10 mg/L Brij 35 within 8 d, which was nearly 3-fold of that in the control (24.6%). Mechanism exploration revealed that the bioavailability of NP and specific NP-degrading bacteria and their functional genes were advantageous to NP biodegradation with alkaline pH and surfactant. More importantly, acidogenic bacteria, the dominant functional bacteria in WAS fermentation systems, were demonstrated to be involved in NP anaerobic biodegradation by providing intermediate organic substrates, as well as through their intrinsic NP-degrading abilities. Possible pathways of NP biodegradation assisted by acidogenic bacteria during anaerobic fermentation were also proposed based on the detected metabolites.
壬基酚（NP）在厌氧条件下难以生物降解。本研究主要通过调控产酸菌在厌氧发酵废活性污泥（WAS）产短链脂肪酸过程中的作用，来提高 NP 的厌氧生物降解。在 pH 值 10.0 和 10mg/L 布罗克菲尔德 Brij 35 条件下，NP 的最大降解效率（69.4%）在 8d 内达到，是对照组（24.6%）的近 3 倍。通过机制探索发现，NP 的生物利用度以及特定的 NP 降解菌及其功能基因有利于碱性 pH 值和表面活性剂条件下的 NP 生物降解。更重要的是，作为 WAS 发酵系统中占主导地位的功能菌，产酸菌通过提供中间有机底物以及其内在的 NP 降解能力，被证明参与了 NP 的厌氧生物降解。基于检测到的代谢物，还提出了厌氧发酵过程中产酸菌辅助 NP 生物降解的可能途径。
