Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, 47000, Selangor, Malaysia.
Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak (UNIMAS), 94300 Kota Samarahan, Sarawak, Malaysia; Institute for Pathology, Laboratory and Forensic Medicine (IPPerForM), Universiti Teknologi MARA, Sungai Buloh Campus, 47000, Selangor, Malaysia.
Life Sci. 2018 Oct 1;210:214-223. doi: 10.1016/j.lfs.2018.08.057. Epub 2018 Aug 24.
Phytoestrogens and xenoestrogens act as agonists/antagonists in bone formation and differentiation. Strong bones are depending of the ability of osteoblasts to form new tissue and to mineralize the newly formed tissue. Dysfunctional or loss of mineralization leads to weak bone and increased fracture risk. In this study, we reported the effect of different types of phytoestrogens (daidzein, genistein and equol) on mineralization in hFOB 1.19 cells stimulated with bisphenol A (BPA).
Cell mineralization capacity of phytoestrogens was investigated by evaluating calcium, phosphate content and alkaline phosphatase activity. Bone related markers, osteocalcin and osteonectin, responsible in maintaining mineralization were also measured.
BPA is significantly interfering with bone mineralization in hFOB 1.19 cells. However, the enhanced mineralization efficacy of daidzein and genistein (particularly at a dose of 5 and 40 μg/mL, respectively) was evidenced by increasing calcium and phosphate content, higher ALP activity, compared to the untreated BPA group. The quantitative analyses were confirmed through morphological findings. Osteocalcin and osteonectin levels were increased in phytoestrogens-treated cells. These findings revealed the potential effect of phytoestrogens in reverting the demineralization process due to BPA exposure in hFOB 1.19 cells.
We found that osteoblast differentiation and mineralization were maintained following treatment with phytoestrogens under BPA exposure.
植物雌激素和外源性雌激素在骨形成和分化中作为激动剂/拮抗剂发挥作用。强壮的骨骼依赖于成骨细胞形成新组织和矿化新形成组织的能力。矿化功能障碍或丧失会导致骨骼脆弱和骨折风险增加。在这项研究中，我们报告了不同类型的植物雌激素（大豆苷元、染料木黄酮和雌马酚）对双酚 A（BPA）刺激的 hFOB 1.19 细胞矿化的影响。
通过评估钙、磷酸盐含量和碱性磷酸酶活性来研究植物雌激素的细胞矿化能力。还测量了负责维持矿化的骨相关标志物骨钙素和骨桥蛋白。
BPA 显著干扰 hFOB 1.19 细胞的骨矿化。然而，大豆苷元和染料木黄酮（特别是在 5 和 40μg/mL 的剂量下）增强了矿化效果，表现为钙和磷酸盐含量增加，碱性磷酸酶活性更高，与未处理的 BPA 组相比。通过形态学发现证实了定量分析。在植物雌激素处理的细胞中，骨钙素和骨桥蛋白水平增加。这些发现揭示了植物雌激素在逆转由于 BPA 暴露而导致的 hFOB 1.19 细胞脱矿化过程中的潜在作用。
我们发现，在 BPA 暴露下，成骨细胞分化和矿化在植物雌激素治疗后得到维持。
