Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, Spain.
Institute of Biosanitary Research, ibs.Granada, Avda. de Madrid, 15 Pabellón de Consultas Externas, 2ª Planta, 18012 Granada, Spain.
Int J Mol Sci. 2022 May 11;23(10):5349. doi: 10.3390/ijms23105349.
(1) Background: Bisphenol A (BPA) is an endocrine disruptor that is widely present in the environment and exerts adverse effects on various body tissues. The objective of this study was to determine its repercussions on bone tissue by examining its impact on selected functional parameters of human osteoblasts. (2) Methods: Three human osteoblast lines were treated with BPA at doses of 10, 10, or 10 M. At 24 h post-treatment, a dose-dependent inhibition of cell growth, alkaline phosphatase activity, and mineralization was observed. (4) Results: The expression of CD54 and CD80 antigens was increased at doses of 10 and 10 M, while the phagocytic capacity and the expression of osteogenic genes (ALP, COL-1, OSC, RUNX2, OSX, BMP-2, and BMP-7) were significantly and dose-dependently reduced in the presence of BPA. (5) Conclusions: According to these findings, BPA exerts adverse effects on osteoblasts by altering their differentiation/maturation and their proliferative and functional capacity, potentially affecting bone health. Given the widespread exposure to this contaminant, further human studies are warranted to determine the long-term risk to bone health posed by BPA.
(1) 背景：双酚 A（BPA）是一种环境内分泌干扰物，广泛存在于环境中，对各种身体组织都有不良影响。本研究旨在通过研究其对人成骨细胞某些功能参数的影响，确定其对骨组织的影响。(2) 方法：用 BPA 处理三种人成骨细胞系，剂量分别为 10、10 或 10 M。处理 24 小时后，观察到细胞生长、碱性磷酸酶活性和矿化的剂量依赖性抑制。(4) 结果：10 和 10 M 剂量下 CD54 和 CD80 抗原的表达增加，而在 BPA 存在下，吞噬能力和成骨基因（ALP、COL-1、OSC、RUNX2、OSX、BMP-2 和 BMP-7）的表达显著且剂量依赖性降低。(5) 结论：根据这些发现，BPA 通过改变成骨细胞的分化/成熟以及增殖和功能能力对其产生不良影响，可能会影响骨骼健康。鉴于这种污染物的广泛暴露，需要进一步的人类研究来确定 BPA 对骨骼健康的长期风险。
