Thent Zar Chi, Froemming Gabriele Ruth Anisah, Ismail Aletza Binti Mohd, Fuad Syed Baharom Syed Ahmad, Muid Suhaila
Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, 47000 Selangor, Malaysia.
Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak (UNIMAS), 94300 Kota Samarahan, Sarawak, Malaysia.
Iran J Basic Med Sci. 2020 Sep;23(9):1155-1163. doi: 10.22038/ijbms.2020.45296.10545.
Since bisphenol A (BPA) induces bone loss and phytoestrogens enhance the osteoblastogenesis by binding to the non-classical and classical oestrogen receptors, respectively, the present study was aimed to observe the osteoprotective effect of phytoestrogens on BPA-induced osteoblasts in hFOB 1.19 cells.
All groups of hFOB 1.19 cells were induced with 12.5 μg/ml of BPA except the control (Ctrl) group. Meanwhile, treated groups received phytoestrogens; Daidzein (Dz), Genistein (Gt), Equol (Eq) and 17β-oestradiol (Est) in different concentrations for 24 hr duration.
We found that the protein expression of non-classical oestrogen-related receptor (ERRG) was highly expressed in BPA group, whereas classical oestrogen receptor alpha (ERα) and oestrogen receptor beta (ERβ) were relatively increased with phytoestrogens treatment under BPA exposure. The dense actin cytoskeletal filaments were also observed. qRT-PCR showed up-regulation of mitogen-activated protein kinase 3 (MAPK3) and G protein-coupled receptor 30 (GPR30) expressions; significant down-regulation of ERRG and up-regulation of ERα and ERβ were observed in phytoestrogens-treated cells, which was supported by the increased expressions of oestrogen receptor 1 (ESR1) and oestrogen receptor 2 (ESR2).
Phytoestrogens improved the deteriorative effect of BPA via down-regulation of ERRG in hFOB 1.19 cells. This study showed that the efficacy of consumption of phytoestrogens in rendering them as potential therapeutic strategy in combating the adverse bone effects of BPA.
由于双酚A（BPA）会导致骨质流失，而植物雌激素分别通过与非经典雌激素受体和经典雌激素受体结合来增强成骨细胞生成，因此本研究旨在观察植物雌激素对hFOB 1.19细胞中BPA诱导的成骨细胞的骨保护作用。
除对照组（Ctrl）外，所有hFOB 1.19细胞组均用12.5μg/ml的BPA诱导。同时，处理组接受不同浓度的植物雌激素；大豆苷元（Dz）、染料木黄酮（Gt）、雌马酚（Eq）和17β-雌二醇（Est），处理24小时。
我们发现，非经典雌激素相关受体（ERRG）的蛋白表达在BPA组中高表达，而在BPA暴露下，植物雌激素处理后经典雌激素受体α（ERα）和雌激素受体β（ERβ）相对增加。还观察到密集的肌动蛋白细胞骨架丝。qRT-PCR显示丝裂原活化蛋白激酶3（MAPK3）和G蛋白偶联受体30（GPR30）表达上调；在植物雌激素处理的细胞中观察到ERRG显著下调，ERα和ERβ上调，雌激素受体1（ESR1）和雌激素受体2（ESR2）表达增加也支持了这一点。
植物雌激素通过下调hFOB 1.19细胞中的ERRG改善了BPA的恶化作用。本研究表明，食用植物雌激素作为对抗BPA对骨骼不良影响的潜在治疗策略具有有效性。
