Department of Legal and Forensic Medicine, University of Murcia, Spain.
Department of Legal and Forensic Medicine, University of Murcia, Spain.
J Proteomics. 2019 Feb 10;192:54-63. doi: 10.1016/j.jprot.2018.08.008. Epub 2018 Aug 23.
Proteomic techniques in bones forensic samples are increasingly, being applied. The main aim of forensic sciences is the estimation of postmortem interval. Most current techniques are useful for the first post-mortem stages. However, in the case of osseous remains, these techniques may be difficult to use due to the high level of decomposition of the sample. Our objective was to attempt to know whether there is a protein profile in human bone remains that would enable a late postmortem. interval ranging from 5 to 20 years postmortem to be estimated. A total of 40 femur bones from 40 different cadavers (data range 5-20 years) were use. Of the 275 total proteins, we excluded the circulating ones (n = 227), leaving a total of 48 proteins (29 structural and 19 functional) were found. A multiple correspondence analysis was applied on the 48 proteins. Finally selecting 32 proteins that allowed us to discriminate between the. two groups of postmortem interval. Analysis of the protein profile present in bone permits an approximation of the date of death within the studied interval, and could be used to complement other tests for estimating the postmortem interval.
骨法医样本中的蛋白质组学技术正日益得到应用。法医学的主要目的是估计死后时间。大多数当前的技术对于死后的早期阶段是有用的。然而，在骨骼遗骸的情况下，由于样本的高度分解，这些技术可能难以使用。我们的目的是试图了解是否存在一种蛋白质图谱，使我们能够估计死后 5 至 20 年的晚期死后时间。总共使用了 40 个来自 40 个不同尸体的股骨（数据范围为 5-20 年）。在 275 种总蛋白质中，我们排除了循环蛋白（n=227），剩下 48 种蛋白质（29 种结构蛋白和 19 种功能蛋白）。对这 48 种蛋白质进行了多次对应分析。最后，选择了 32 种蛋白质，可以区分两个死后间隔组。对骨骼中存在的蛋白质图谱进行分析，可以估计研究间隔内的死亡日期，并可用于补充其他估计死后时间的测试。
