The Forensic Science Unit, Faculty of Health and Life Sciences, Ellison Building, Northumbria University, Newcastle upon Tyne NE1 8ST, U.K.
J Proteome Res. 2020 May 1;19(5):2122-2135. doi: 10.1021/acs.jproteome.0c00060. Epub 2020 Apr 16.
Methods currently available to estimate the submerged interval (PMSI) of cadavers in water suffer from poor accuracy, being mostly based on morphological examination of the remains. Proteins present within bones have recently attracted more attention from researchers interested in the estimation of the interval (PMI) in terrestrial environments. Despite the great potential of proteomic methods for PMI estimation, their application to aquatic environments has not yet been explored. In this study, we examined whether four different types of aquatic environment affected the proteome of mice bones with increasing PMSIs. Results showed that increasing PMSIs can influence the protein abundances more than the different types of water. In particular, the abundance of the muscle protein fructose-bisphosphate aldolase A constantly decreased with increasing PMSIs. Additionally, the protein peptidyl-prolyl cis-trans isomerase showed a significant decrease between controls and aquatic environments. Furthermore, the coagulation factor VII was deamidated only in submerged samples and not in terrestrial controls. Finally, fetuin-A was significantly more deamidated in pond water compared to the other aquatic environments. Overall, this study identified novel potential biomarker candidates that would be useful for the estimation of the PMSI and for the characterization of the type of water involved in criminal investigations.
方法目前可用于估计在水中的尸体的淹没间隔（PMSI）遭受准确性差，大多基于对遗骸的形态检查。 最近，在研究人员对陆地环境中的间隔（PMI）的估计感兴趣的蛋白质存在于骨骼中吸引了更多的关注。 尽管蛋白质组学方法在 PMI 估计中有很大的潜力，但它们在水生环境中的应用尚未得到探索。 在这项研究中，我们检查了四种不同类型的水生环境是否会影响随着 PMSI 增加的小鼠骨骼的蛋白质组。 结果表明，随着 PMSI 的增加，蛋白质的丰度比不同类型的水更能影响蛋白质的丰度。 具体而言，随着 PMSI 的增加，肌肉蛋白果糖二磷酸醛缩酶 A 的丰度不断下降。 此外，肽基脯氨酰顺反异构酶的蛋白质在对照和水生环境之间显示出显著降低。 此外，凝血因子 VII 仅在淹没样品中脱酰胺，而在陆地对照中则不脱酰胺。 最后，胎球蛋白-A 在池塘水中的脱酰胺明显多于其他水生环境。 总的来说，这项研究确定了新的潜在生物标志物候选物，这将有助于估计 PMSI，并有助于描述犯罪调查中涉及的水的类型。
