[利用不连续梯度离心法从人外周血中分离淋巴细胞类别]
[Separation of lymphocytes into classes from peripheral human blood using centrifugation on a discontinuous gradient].
作者信息
Hadzija M, Poljak-Blazi M, Boranić M
出版信息
Bilt Hematol Transfuz. 1978;6(1):13-21.
PMID:314801
Abstract
Peripheral blood lymphocytes of 20 normal volunteers were separated on a discontinuous gradient of Ficoll. The ability of lymphocytes from the fractions to from sponteneous (E) and induced (EAC) rosettes and undergo transformation in the presence of phytohaemagglutinin (PHA) was tested. T-lymphocytes were concentrated in the density of 1,067 g/ml and B-lymphocytes in the density of 1,063 g/ml.
摘要
从20名正常志愿者的外周血淋巴细胞中，通过不连续的Ficoll梯度进行分离。检测了各组分淋巴细胞形成自发（E）和诱导（EAC）玫瑰花结以及在植物血凝素（PHA）存在下发生转化的能力。T淋巴细胞集中在密度为1.067 g/ml处，B淋巴细胞集中在密度为1.063 g/ml处。
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