Department of Orthopedics, The Third Affiliated Hospital of Nanchang University.
Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University.
Biosci Trends. 2018 Sep 19;12(4):389-394. doi: 10.5582/bst.2018.01117. Epub 2018 Aug 27.
Long non-coding RNA taurine-upregulated gene 1 (lncRNA TUG1) promotes osteosarcoma, while its involvement in other bone diseases, such as ankylosing spondylitis (AS) is unknown. Expression of TUG1 in serum and open sacroiliac biopsies of AS patents and healthy controls was detected by real-time quantitative PCR (qRT-PCR). Ankylosing spondylitis disease activity score (ASDAS) system was used to evaluate disease activity. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic value of lncRNA TUG1 for AS. Chi-square test was performed to analyze the correlations between TUG1 expression and patients' clinicopathological data. Patients were divided into 2 groups (high and low expression groups) according to the median expression level of TUG1 and were followed-up for 5 years after discharge. Treatment courses and rehospitalization rate were compared between two groups. It was observed that TUG1 expression level was significantly lower in AS patients than in healthy controls in both serum and biopsies. Reduced expression level of TUG1 distinguished AS patients from controls. LncRNA TUG1 expression was significantly correlated with patients' smoking habits, disease activity, and course of disease. Patients in high expression group showed longer hospitalization time and higher rehospitalization rate. We therefore conclude that expression of lncRNA TUG1 was inhibited in AS patients and downregulation of lncRNA TUG1 is related to higher disease activity, longer course of treatment and higher rehospitalization rate.
长链非编码 RNA 牛磺酸上调基因 1（lncRNA TUG1）促进骨肉瘤的发生，但其在其他骨病（如强直性脊柱炎（AS））中的作用尚不清楚。通过实时定量 PCR（qRT-PCR）检测 AS 患者和健康对照者血清和开放骶髂活检中 TUG1 的表达。采用强直性脊柱炎疾病活动评分（ASDAS）系统评估疾病活动度。采用受试者工作特征（ROC）曲线分析评估 lncRNA TUG1 对 AS 的诊断价值。采用卡方检验分析 TUG1 表达与患者临床病理资料的相关性。根据 TUG1 的中位表达水平将患者分为 2 组（高表达组和低表达组），并在出院后随访 5 年。比较两组患者的治疗疗程和再住院率。结果发现，血清和活检中 AS 患者的 TUG1 表达水平均明显低于健康对照组。TUG1 表达水平降低可将 AS 患者与对照组区分开来。lncRNA TUG1 表达与患者的吸烟习惯、疾病活动度和病程显著相关。高表达组患者的住院时间更长，再住院率更高。因此，我们得出结论，AS 患者的 lncRNA TUG1 表达受到抑制，lncRNA TUG1 的下调与更高的疾病活动度、更长的治疗疗程和更高的再住院率相关。
