Li Xiang, Chai Wei, Zhang Guoqiang, Ni Ming, Chen Jiying, Dong Jiyuan, Zhou Yonggang, Hao Libo, Bai Yang, Wang Yan
Department of Orthopaedics, PLA General Hospital, Beijing, China (mainland).
Department of Stomatology, PLA General Hospital, Beijing, China (mainland).
Med Sci Monit. 2017 Jan 2;23:11-16. doi: 10.12659/msm.898915.
BACKGROUND Long non-coding RNAs (lncRNAs) have been confirmed to play an important role in the development and progression of diseases. Ankylosing spondylitis (AS) is a chronic inflammatory systemic disease and it is hard to be found in early time. The purpose of this study was to investigate the role of lncRNA-AK001085 in the diagnosis of AS. MATERIAL AND METHODS The expression of lncRNA-AK001085 was detected by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The relationship between its expression and clinicopathologic characteristics was also analyzed. Meanwhile the correlation between lncRNA-AK001085 expression and diseases activity indexes was estimated. In addition, the value of it in the diagnosis of AS was explored through establishing receiver operating characteristic (ROC) curve. RESULTS Serum lncRNA-AK001085 expression was decreased in patients with AS compared with healthy individuals. And its expression was proved to be influenced by ever cigarette smoker, exercise level and occupational activity level. Besides, the correlation of the expression of lncRNA-AK001085 and disease activity indexes (BASDI, ASDAS, ESR, CRP) were all negative, which suggested that the lncRNA-AK001085 was significantly lower in patients with a high disease activity score. It might showed that the expression of lncRNA-AK001085 affected the activity of AS. CONCLUSIONS LncRNA-AK001085 was down-regulated in AS patients and it could be an independent diagnostic indicator.
背景 长链非编码RNA（lncRNAs）已被证实在疾病的发生发展过程中发挥重要作用。强直性脊柱炎（AS）是一种慢性炎症性全身性疾病，早期难以发现。本研究旨在探讨lncRNA-AK001085在AS诊断中的作用。
材料与方法 采用定量实时聚合酶链反应（qRT-PCR）分析检测lncRNA-AK001085的表达。分析其表达与临床病理特征之间的关系。同时评估lncRNA-AK001085表达与疾病活动指标之间的相关性。此外，通过建立受试者工作特征（ROC）曲线探讨其在AS诊断中的价值。
结果 与健康个体相比，AS患者血清lncRNA-AK001085表达降低。其表达受既往吸烟情况、运动水平和职业活动水平的影响。此外，lncRNA-AK001085表达与疾病活动指标（BASDI、ASDAS、ESR、CRP）均呈负相关，这表明疾病活动评分高的患者lncRNA-AK001085显著降低。这可能表明lncRNA-AK001085的表达影响了AS 的活动。
结论 lncRNA-AK001085在AS患者中表达下调，可能是一个独立的诊断指标。
