Bin Masalam Maged S, Bahieldin Ahmed, Alharbi Mona G, Al-Masaudi Saad, Al-Jaouni Soad K, Harakeh Steve M, Al-Hindi Rashad R
Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Department of Pediatric Hematology/Oncology, Yousef Abdullatif Jameel Chair of Prophetic Medicine Application, King Abdulaziz University Hospital, Faculty of Medicine, KAU, Saudi Arabia.
Evid Based Complement Alternat Med. 2018 Jul 25;2018:7970463. doi: 10.1155/2018/7970463. eCollection 2018.
Probiotic bacteria can confer health benefits to the human gastrointestinal tract. Lactic acid bacteria (LAB) are candidate probiotic bacteria that are widely distributed in nature and can be used in the food industry. The objective of this study is to isolate and characterize LAB present in raw and fermented milk in Saudi Arabia. Ninety-three suspected LAB were isolated from thirteen different types of raw and fermented milk from indigenous animals in Saudi Arabia. The identification of forty-six selected LAB strains and their genetic relatedness was performed based on 16S rDNA gene sequence comparisons. None of the strains exhibited resistance to clinically relevant antibiotics or had any undesirable hemolytic activity, but they differed in their other probiotic characteristics, that is, tolerance to acidic pH, resistance to bile, and antibacterial activity. In conclusion, the isolates MSJ1, Dwan5, EyLan2, and Gail-BawZir8 are most likely the best with probiotic potentials. We speculate that studying the synergistic effects of bacterial combinations might result in a more effective probiotic potential. We suspect that raw and fermented milk products from animals in Saudi Arabia, especially Laban made from camel milk, are rich in LAB and have promising probiotic potential.
益生菌可对人类胃肠道产生有益健康的作用。乳酸菌（LAB）是候选益生菌，广泛分布于自然界，可用于食品工业。本研究的目的是分离并鉴定沙特阿拉伯生乳和发酵乳中存在的乳酸菌。从沙特阿拉伯本土动物的13种不同类型的生乳和发酵乳中分离出93株疑似乳酸菌。基于16S rDNA基因序列比较对46株选定的乳酸菌菌株进行鉴定及其亲缘关系分析。所有菌株均未表现出对临床相关抗生素的抗性或具有任何不良溶血活性，但它们在其他益生菌特性方面存在差异，即耐酸性pH、耐胆汁性和抗菌活性。总之，分离株MSJ1、Dwan5、EyLan2和Gail - BawZir8最有可能具有最佳的益生菌潜力。我们推测研究细菌组合的协同效应可能会产生更有效的益生菌潜力。我们怀疑沙特阿拉伯动物的生乳和发酵乳制品，尤其是骆驼奶制成的拉班，富含乳酸菌且具有良好的益生菌潜力。
