Moss S R, Nuttall P A
Virus Res. 1986 Jun;4(4):331-6. doi: 10.1016/0168-1702(86)90079-1.
Eighteen stable temperature sensitive (ts) mutants of Broadhaven virus were isolated without the aid of mutagens. Spontaneous mutants were detected using 41 degrees C as the nonpermissive temperature and 36 degrees C as the permissive temperature. High frequency pairwise recombination defined five recombination groups. Four mutants belonged to group I, three to group II, six to group III, two to group IV, and two to group V. ts 7 was a possible double mutant representing lesions corresponding to those of groups III and V mutants. This is the first reported isolation of temperature sensitive mutants of a tick-borne orbivirus.
在未借助诱变剂的情况下，分离出了18株布罗德黑文病毒的稳定温度敏感（ts）突变体。利用41℃作为非允许温度、36℃作为允许温度来检测自发突变体。高频成对重组确定了五个重组组。四个突变体属于I组，三个属于II组，六个属于III组，两个属于IV组，两个属于V组。ts 7可能是一个双突变体，代表了与III组和V组突变体相对应的损伤。这是首次报道蜱传环状病毒温度敏感突变体的分离。
