Phule Amol S, Barbadikar Kalyani M, Madhav M S, Senguttuvel P, Babu M B B Prasad, Ananda Kumar P
1Biotechnology Division, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, 500030 Telangana India.
2Institute of Biotechnology, Professor Jayashankar Telangana State Agricultural University, Hyderabad, 500030 Telangana India.
3 Biotech. 2018 Sep;8(9):383. doi: 10.1007/s13205-018-1406-9. Epub 2018 Aug 23.
A set of reference genes expressing stably under aerobic and anaerobic conditions in rice is essential to execute omics studies relating to aerobic adaptations. Stability of expression of ten rice reference genes, viz. , and was validated across six experimental sets in shoot and root tissues at seedling, tillering, and panicle initiation stages. Comprehensively, (Membrane protein), (Tumor protein homolog), and (Expressed protein) were revealed as the most stable set with acceptable and value according to the gold standards of qRT-PCR using various algorithms/tools. The identified set of reference genes was validated using root trait genes, which showed concurrence with the functional expression patterns in the aerobic and anaerobic adapted cultivars. The (Membrane protein), (Tumor protein homolog), and (Expressed protein) genes are the most stable reference genes across the root and shoot at various developmental stages under aerobic and anaerobic conditions in rice. This is the first study for accurate and reliable relative gene expression analysis in rice grown in aerobic and anaerobic conditions.
一组在水稻有氧和无氧条件下稳定表达的参考基因对于开展与有氧适应性相关的组学研究至关重要。对十个水稻参考基因（即 、 、 、 、 、 、 、 、 、 ）在幼苗期、分蘖期和幼穗分化期的地上部和根组织的六个实验组中的表达稳定性进行了验证。综合来看，根据使用各种算法/工具的qRT-PCR金标准， （膜蛋白）、 （肿瘤蛋白同源物）和 （表达蛋白）被揭示为最稳定的一组，其 和 值可接受。使用根性状基因对鉴定出的参考基因集进行了验证，结果表明其与有氧和无氧适应品种中的功能表达模式一致。 （膜蛋白）、 （肿瘤蛋白同源物）和 （表达蛋白）基因是水稻在有氧和无氧条件下不同发育阶段地上部和根中最稳定的参考基因。这是首次针对在有氧和无氧条件下生长的水稻进行准确可靠的相对基因表达分析的研究。
