Bevitori R, Oliveira M B, Grossi-de-Sá M F, Lanna A C, da Silveira R D, Petrofeza S
Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, Brasil.
Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
Genet Mol Res. 2014 Nov 27;13(4):9795-805. doi: 10.4238/2014.November.27.7.
Drought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α> β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4αgenes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice.
干旱和由稻瘟病菌引起的稻瘟病是对全球水稻生产最严重的两大威胁。为探究胁迫诱导水稻基因表达的潜在机制，过去几年开展了涉及转录组分析的研究。因此，拥有一套可靠的内参基因以标准化受不同胁迫影响的水稻基因的表达水平至关重要。为鉴定在稻瘟病菌感染和干旱条件下水稻中靶基因差异表达研究的潜在内参基因，本研究评估了五个管家基因用于基因表达的标准化。使用geNorm和NormFinder分析软件包评估这些基因表达的稳定性。对于所有分析的样本，稳定性排名为UBQ5 > GAPDH > eIF-4α> β-TUB > 18S rRNA。数据表明，UBQ5、GAPDH和eIF-4α基因是合适的、高性能的内参基因，在未来水稻真菌感染和干旱的表达研究中将非常有用。
