Jeon Tina, Vutescu Emil S, Saltzman Eliana B, Villa Jordan C, Wolfe Scott W, Lee Steve K, Feinberg Joseph H, Pownder Sarah L, Dyke Jonathan P, Sneag Darryl B
1Department of Radiology and Imaging, Hospital for Special Surgery, 535 E 70th Street, New York, NY 10021 USA.
2Department of Hand and Upper Extremity Service, Hospital for Special Surgery, 535 E 70th Street, New York, NY 10021 USA.
Eur Radiol Exp. 2018 Aug 8;2:19. doi: 10.1186/s41747-018-0049-2. eCollection 2018 Dec.
We compared different surgical techniques for nerve regeneration in a rabbit sciatic nerve gap model using magnetic resonance diffusion tensor imaging (DTI), electrophysiology, limb function, and histology.
A total of 24 male New Zealand white rabbits were randomized into three groups: autograft ( = 8), hollow conduit ( = 8), and collagen-filled conduit ( = 8). A 10-mm segment of the rabbit proximal sciatic nerve was cut, and autograft or collagen conduit was used to bridge the gap. DTI on a 3-T system was performed preoperatively and 13 weeks after surgery using the contralateral, nonoperated nerve as a control.
Overall, autograft performed better compared with both conduit groups. Differences in axonal diameter were significant (autograft > hollow conduit > collagen-filled conduit) at 13 weeks (autograft vs. hollow conduit, = 0.001, and hollow conduit vs. collagen-filled conduit, < 0.001). Significant group differences were found for axial diffusivity but not for any of the other DTI metrics (autograft > hollow conduit > collagen-filled conduit) (autograft vs. hollow conduit, = 0.001 and hollow conduit vs. collagen-filled conduit, = 0.021). As compared with hollow conduit (autograft > collagen-filled conduit > hollow conduit), collagen-filled conduit animals demonstrated a nonsignificant increased maximum tetanic force.
Autograft-treated rabbits demonstrated improved sciatic nerve regeneration compared with collagen-filled and hollow conduits as assessed by histologic, functional, and DTI parameters at 13 weeks.
我们在兔坐骨神经缺损模型中，使用磁共振扩散张量成像（DTI）、电生理学、肢体功能和组织学方法，比较了不同的神经再生手术技术。
将24只雄性新西兰白兔随机分为三组：自体移植组（n = 8）、空心导管组（n = 8）和胶原填充导管组（n = 8）。切断兔坐骨神经近端10毫米的一段，使用自体移植或胶原导管桥接缺损。术前及术后13周在3-T系统上进行DTI检查，以对侧未手术的神经作为对照。
总体而言，自体移植组的表现优于两个导管组。术后13周时，轴突直径差异显著（自体移植组>空心导管组>胶原填充导管组）（自体移植组与空心导管组比较，P = 0.001；空心导管组与胶原填充导管组比较，P < 0.001）。轴向扩散率存在显著的组间差异，但其他DTI指标均无差异（自体移植组>空心导管组>胶原填充导管组）（自体移植组与空心导管组比较，P = 0.001；空心导管组与胶原填充导管组比较，P = 0.021）。与空心导管组相比（自体移植组>胶原填充导管组>空心导管组），胶原填充导管组动物的最大强直收缩力有不显著增加。
在术后13周通过组织学、功能和DTI参数评估，自体移植治疗的兔坐骨神经再生情况优于胶原填充导管组和空心导管组。
