Yadav Aditya, Rao Chethana, Nandi Chayan K
School of Basic Sciences, Indian Institute of Technology Mandi, Mandi 175075, H.P., India.
Advanced Materials Research Centre, Indian Institute of Technology Mandi, Mandi 175075, H.P., India.
ACS Omega. 2020 Oct 13;5(42):26967-26977. doi: 10.1021/acsomega.0c04018. eCollection 2020 Oct 27.
Lysosomes are membrane-enclosed small spherical cytoplasmic organelles. Malfunctioning and abnormalities in lysosomes can cause a plethora of neurodegenerative diseases. Consequently, understanding the structural information on lysosomes down to a subnanometer level is essential. Recently, super-resolution imaging techniques enable us to visualize dynamical processes occurring in suborganelle structures inside living cells down to subnanometer accuracy by breaking the diffraction limit. A brighter and highly photostable fluorescent probe is essential for super-resolution microscopy. In this regard, this mini-review deals with the various types of super-resolution techniques and the probes that are used to specifically stain and resolve the structure of the lysosomes.
溶酶体是由膜包裹的小型球形细胞质细胞器。溶酶体功能失调和异常可导致多种神经退行性疾病。因此，深入了解溶酶体亚纳米级别的结构信息至关重要。最近，超分辨率成像技术能够突破衍射极限，以亚纳米精度可视化活细胞内亚细胞器结构中发生的动态过程。对于超分辨率显微镜而言，更亮且光稳定性高的荧光探针至关重要。在这方面，本综述介绍了各种超分辨率技术以及用于特异性标记和解析溶酶体结构的探针。
