Schoeller D A, Kushner R F, Jones P J
Am J Clin Nutr. 1986 Aug;44(2):291-8. doi: 10.1093/ajcn/44.2.291.
The doubly labeled water method was compared with intake-balance for measuring energy expenditure in five patients receiving total parenteral nutrition (TPN). Because parenteral solutions were isotopically different from local water, patients had to be placed on TPN at least 10 days before the metabolic period. Approximately 0.1 g 2H2O and 0.25 g H2(18)O per kg total body water were given orally. We collected saliva before, 3 h, and 4 h after the dose for measurement of total body water and urine before, 1 day, and 14 days after the dose for measurement of isotope eliminations. On day 14, total body weight was remeasured and change in body energy stores was calculated, assuming constant hydration. Intake was assessed from weights of TPN fluids plus dietary record for any oral intake. Energy expenditure from doubly labeled water (+/- SD) averaged 3 +/- 6% greater than intake-balance. Doubly labeled water method is a noninvasive, nonrestrictive method for measuring energy expenditure in patients receiving TPN.
对5名接受全胃肠外营养（TPN）的患者，比较了双标记水法与摄入量平衡法在测量能量消耗方面的差异。由于胃肠外营养液与当地的水在同位素方面存在差异，患者必须在代谢期前至少10天开始接受TPN。按每千克总体水口服给予约0.1 g 2H2O和0.25 g H2(18)O。给药前、给药后3小时和4小时收集唾液以测量总体水，给药前、给药后1天和14天收集尿液以测量同位素消除情况。在第14天，重新测量总体重，并在假设水合作用恒定的情况下计算身体能量储存的变化。摄入量通过TPN液体重量加上任何口服摄入量的饮食记录进行评估。双标记水法测得的能量消耗（±标准差）平均比摄入量平衡法高3±6%。双标记水法是一种用于测量接受TPN患者能量消耗的非侵入性、非限制性方法。
