高比活度D-[3-³H]泛酸的制备

Preparation of high-specific-activity D-[3-3H]pantothenic acid.

作者信息

Vallari D S, Rock C O

出版信息

Anal Biochem. 1986 May 1;154(2):671-5. doi: 10.1016/0003-2697(86)90045-x.

Abstract

High-specific-activity D-[3-3H]pantothenic acid (5 Ci/mmol) was prepared from commercially available beta-[3-3H]alanine employing Escherichia coli strain DV1 (panD2 pan F1). This strain is defective in beta-alanine synthesis and pantothenate uptake, and under appropriate growth conditions converted 85 to 90% of the input beta-[3-3H]alanine to extracellular D-[3-3H]pantothenate. The radiolabeled vitamin was purified from the medium by thin-layer chromatography followed by reverse-phase high-performance liquid chromatography. The overall yield of D-[3-3H]pantothenic acid was 30% and radiochemical purity was greater than 99%.

摘要

高比活度的D-[3-³H]泛酸（5 Ci/mmol）由市售的β-[3-³H]丙氨酸，利用大肠杆菌DV1菌株（panD2 pan F1）制备。该菌株在β-丙氨酸合成和泛酸盐摄取方面存在缺陷，在适当的生长条件下，能将85%至90%的输入β-[3-³H]丙氨酸转化为细胞外D-[3-³H]泛酸盐。通过薄层色谱，然后进行反相高效液相色谱，从培养基中纯化出放射性标记的维生素。D-[3-³H]泛酸的总产率为30%，放射化学纯度大于99%。