Research Center for Bioengineering & Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Research Center for Bioengineering & Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China; Key Laboratory of Cosmetic (Beijing Technology and Business University), China National Light Industry, Beijing 100048, China.
Colloids Surf B Biointerfaces. 2018 Dec 1;172:161-169. doi: 10.1016/j.colsurfb.2018.08.014. Epub 2018 Aug 10.
The accumulation of bilirubin in the body could cause nervous system diseases and even endanger life in severe cases for people with liver damage or metabolic obstruction. Hemoperfusion has been considered as one of the most efficient treatments to remove extra bilirubin. Although, the current bilirubin adsorbents could adsorb the free bilirubin effectively, the albumin-bound bilirubin in plasma is hard to remove. Here, we develop a 3D nanofiber sponge fabricated by combination of electrospinning and improved gas-foaming techniques. The amino groups and BSA molecules were immobilized on the fiber surface as the affinity groups to adsorb bilirubin. The 3D nanofiber sponges have layered structure and significantly higher porosity than two-dimensional nanofiber membranes. The special 3D structure renders the sponge fully contact with the adsorbed liquid and reduces the diffusion distance of the adsorbate, thus increases the sponge's adsorption rate. The BSA immobilized nanofiber sponge showed large adsorption capacity in both aqueous solution (maximum adsorption capacity was 36.8237 mg/g) and plasma (maximum adsorption capacity was 25.2908 mg/g), rapid adsorption rate (achieved adsorption equilibrium in 60 min) and well blood compatibility.
胆红素在体内的积累可能导致神经系统疾病，甚至在严重的情况下危及肝功能或代谢障碍患者的生命。血液灌流已被认为是去除多余胆红素最有效的治疗方法之一。然而，目前的胆红素吸附剂虽然可以有效地吸附游离胆红素，但血浆中的结合胆红素却难以去除。在这里，我们开发了一种由静电纺丝和改进的气体发泡技术相结合制备的 3D 纳米纤维海绵。纤维表面的氨基和 BSA 分子被固定为亲和基团以吸附胆红素。3D 纳米纤维海绵具有分层结构，比二维纳米纤维膜具有更高的孔隙率。特殊的 3D 结构使海绵与吸附液充分接触，并减少了吸附质的扩散距离，从而提高了海绵的吸附速率。固定化 BSA 的纳米纤维海绵在水溶液（最大吸附容量为 36.8237mg/g）和血浆（最大吸附容量为 25.2908mg/g）中均表现出较大的吸附容量，吸附速率快（60min 内达到吸附平衡），且具有良好的血液相容性。
