Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China.
Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China.
ACS Appl Mater Interfaces. 2020 Jun 10;12(23):25546-25556. doi: 10.1021/acsami.0c03859. Epub 2020 Jun 1.
Adsorption-based hemoperfusion has been widely used to remove toxins from the blood of patients suffering acute liver failure (ALF). However, its detoxification effect has been severely hampered by the unsatisfactory adsorption performance of clinically used porous adsorbents, such as activated carbon (AC) and adsorption resin. Herein, two cage-based metal-organic frameworks (MOFs), PCN-333 (constructed from 4,4,4-s-triazine-2,4,6-triyl-tribenzoic acid (HTATB) ligands and Al metal clusters) and MOF-808 (constructed from 1,3,5-benzenetricarboxylic acid (HBTC) ligands and Zr metal clusters), are introduced for highly efficient hemoperfusion. They possess negligible hemolytic activity and can act as "bilirubin traps" to achieve outstanding adsorption performance toward bilirubin, a typical toxin related to ALF. Notably, PCN-333 shows a record-high adsorption capacity (∼1003.8 mg g) among various bilirubin adsorbents previously reported. More importantly, they can efficiently adsorb bilirubin in bovine serum albumin (BSA) solution or even in 100% fetal bovine serum (FBS) due to their high selectivity. Strikingly, the adsorption rate and capacity of PCN-333 in biological solutions are approximately four times faster and 69 times higher than those of clinical AC, respectively. Findings in this work pave a new avenue to overcome the challenge of low adsorption efficiency and capacity in hemoperfusion therapy.
基于吸附的血液灌流已广泛应用于从急性肝衰竭（ALF）患者的血液中去除毒素。然而，其解毒效果受到临床使用的多孔吸附剂（如活性炭（AC）和吸附树脂）吸附性能不佳的严重阻碍。在此，介绍了两种基于笼状的金属有机骨架（MOF），PCN-333（由 4,4,4-三嗪-2,4,6-三基三苯甲酸（HTATB）配体和 Al 金属簇构建）和 MOF-808（由 1,3,5-苯三甲酸（HBTC）配体和 Zr 金属簇构建），用于高效血液灌流。它们具有可忽略的溶血活性，可以作为“胆红素陷阱”，对胆红素（一种与 ALF 相关的典型毒素）表现出出色的吸附性能。值得注意的是，PCN-333 在以前报道的各种胆红素吸附剂中表现出了创纪录的高吸附容量（约 1003.8 mg g）。更重要的是，由于其高选择性，它们可以有效地吸附牛血清白蛋白（BSA）溶液甚至 100％胎牛血清（FBS）中的胆红素。引人注目的是，PCN-333 在生物溶液中的吸附速率和容量分别比临床 AC 快约 4 倍和高 69 倍。这项工作的结果为克服血液灌流治疗中吸附效率和容量低的挑战开辟了新途径。
