Thomas P M, Kuykendall L D, Angle J S
Appl Environ Microbiol. 1986 Jul;52(1):206-8. doi: 10.1128/aem.52.1.206-208.1986.
Techniques for in vivo cloning were used with the fast-growing nitrogen-fixing soybean microsymbiont R. fredii USDA 191. Selection for transfer of Tn5 insertions from R. fredii USDA 191 containing the gene-mobilizing plasmid pJB3JI provided recombinants at up to 400 times the background mutation level. These techniques may be useful for future genetic analysis of R. fredii.
体内克隆技术被应用于快速生长的固氮大豆微共生体费氏中华根瘤菌USDA 191。从含有基因转移质粒pJB3JI的费氏中华根瘤菌USDA 191中选择转座子Tn5插入的转移，得到的重组体的突变水平比背景突变水平高400倍。这些技术可能对未来费氏中华根瘤菌的遗传分析有用。
