慢性牙周炎患者牙龈卟啉单胞菌的分子检测
Molecular Detection of Porphyromonas gingivalis in Chronic Periodontitis Patients.
作者信息
Kulkarni Pavan G, Gosavi Suchitra, Haricharan Praveen B, Malgikar Suryakanth, Mudrakola Durga P, Turagam Neeraja, Ealla Kranti Kr
机构信息
Department of Oral and Maxillofacial Pathology, Kamineni Institute of Dental Sciences, Hyderabad, Telangana, India.
Department of Oral and Maxillofacial Pathology, Government Dental College & Hospital, Nagpur, Maharashtra, India.
出版信息
J Contemp Dent Pract. 2018 Aug 1;19(8):992-996.
AIM
In the current study, Porphyromonas gingivalis was identified in chronic periodontitis patients and healthy subjects by polymerase chain reaction (PCR) and its presence correlated with the severity of clinical periodontal parameters.
MATERIALS AND METHODS
Subgingival plaque samples were collected with sterile curette and subjected to deoxyribonucleic acid (DNA) extraction and subsequent PCR for detection of P. gingivalis.
RESULTS
Porphyromonas gingivalis was detected in 60% of patients of group II (pocket depth up to 5 mm), and in 93.33% of patients of group III (pocket depth more than 5 mm). One periodontally healthy subject in group I (probing depth < 3 mm) showed the presence of P. gingivalis.
CONCLUSION
Detection frequency of bacterium increased significantly with increase in probing pocket depth (PPD), loss of attachment (LOA), and gingival index (GI).
CLINICAL SIGNIFICANCE
Porphyromonas gingivalis is strongly associated with chronic periodontitis and its detection frequency positively correlates with the severity of periodontal destruction.
目的
在本研究中，通过聚合酶链反应（PCR）在慢性牙周炎患者和健康受试者中鉴定牙龈卟啉单胞菌，并将其存在情况与临床牙周参数的严重程度相关联。
材料与方法
用无菌刮匙采集龈下菌斑样本，进行脱氧核糖核酸（DNA）提取，随后进行PCR检测牙龈卟啉单胞菌。
结果
在II组（牙周袋深度达5mm）60%的患者中检测到牙龈卟啉单胞菌，在III组（牙周袋深度超过5mm）93.33%的患者中检测到。I组（探诊深度<3mm）中一名牙周健康受试者也检测到牙龈卟啉单胞菌。
结论
随着探诊深度（PPD）、附着丧失（LOA）和牙龈指数（GI）增加，该细菌的检出频率显著升高。
临床意义
牙龈卟啉单胞菌与慢性牙周炎密切相关，其检出频率与牙周破坏的严重程度呈正相关。
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