Estruch R, Damjanov I
Arch Pathol Lab Med. 1986 Aug;110(8):730-5.
Histologic sections of normal and pathologically altered human peripheral nerves were stained with a battery of 20 fluorescein isothiocyanate-labeled lectins to determine whether these histochemical reagents could be used to identify peripheral nerve injury. Eight plant lectins--from Canavalia ensiformis, Lens culinaris, Triticum vulgare (wheat germ), types E4 and L4 from Phaseolus vulgaris, types I and II from Ricinus communis, and Wistaria floribunda--were found to bind to normal and pathologically altered nerves. Only two lectins, from Helix pomatia and Maclura pomifera, were found to bind selectively to pathologically altered nerves. The changes recognized by lectin histochemistry were not pathognomonic of any specific type of nerve injury. This study provides baseline data on the reaction pattern of human peripheral nerve with a series of lectins and shows that lectin histochemistry could provide means for the study of peripheral nerve pathology.
用一组20种异硫氰酸荧光素标记的凝集素对正常和病理改变的人周围神经组织切片进行染色，以确定这些组织化学试剂是否可用于识别周围神经损伤。发现来自刀豆、小扁豆、普通小麦（麦芽）、菜豆E4和L4型、蓖麻I和II型以及紫藤的8种植物凝集素可与正常和病理改变的神经结合。仅发现来自苹果蜗牛和桑橙的两种凝集素可选择性地与病理改变的神经结合。凝集素组织化学所识别的变化并非任何特定类型神经损伤的特征性表现。本研究提供了关于人周围神经与一系列凝集素反应模式的基线数据，并表明凝集素组织化学可为周围神经病理学研究提供手段。
