Sato T, Suzuki H, Kitaoka S, Konno T, Ishida N
Arch Virol. 1986;90(1-2):29-40. doi: 10.1007/BF01314142.
Polypeptide analysis of three strains of human rotavirus (KUN, Wa and MO) were conducted using a hypertonic culture which suppressed host protein synthesis and unmasked rotavirus specific protein synthesis. As a result, eleven human rotavirus specific polypeptides (Vp 1--Vp 11) were detected by pulselabeling infected cells with [14C]-leucine. Among the 11 polypeptides, three polypeptides (Vp 7, Vp 10 and Vp 11) underwent post-translational processing, and two (Vp 7 and Vp 10) were glycosylated. Six polypeptides (Vp 1, 2, 3, 4, 6 and 7) were identified as viral structural proteins. Comparisons of three strains of different serotypes revealed that their polypeptide profiles differed from each other in electrophoretic mobility; in particular, profiles of the glycosylated polypeptide, Vp 7, were distinct among the three strains.
利用一种高渗培养方法对三株人轮状病毒（KUN、Wa和MO）进行了多肽分析，该方法可抑制宿主蛋白合成并揭示轮状病毒特异性蛋白合成。结果，通过用[14C] - 亮氨酸脉冲标记感染细胞，检测到了11种人轮状病毒特异性多肽（Vp 1 - Vp 11）。在这11种多肽中，有三种多肽（Vp 7、Vp 10和Vp 11）经历了翻译后加工，其中两种（Vp 7和Vp 10）进行了糖基化。六种多肽（Vp 1、2、3、4、6和7）被鉴定为病毒结构蛋白。对三株不同血清型病毒的比较显示，它们的多肽图谱在电泳迁移率上彼此不同；特别是，糖基化多肽Vp 7的图谱在这三株病毒中明显不同。
