Urasawa T, Urasawa S, Taniguchi K
Microbiol Immunol. 1981;25(10):1025-35. doi: 10.1111/j.1348-0421.1981.tb00109.x.
Starting with a small amount of diarrheal feces containing human rotavirus (HRV), we succeeded in propagation of the virus using the roller culture technique with MA-104 cells. Furthermore, we made a successful adaptation of HRV to a stationary culture and developed a plaque assay for the cell culture-adapted viruses. The 3 culture-adapted virus isolates, KU, YO, and 44 produced plaques (about 0.5-1.0 mm in diameter) under the overlay medium consisting of 0.6% purified agar, 3 micrograms of acetyl trypsin/ml and 50 micrograms of DEAE-dextran/ml. Subsequent plaque purification resulted in the formation of clear, larger plaques. It was shown from the results of cross neutralization tests using the fluorescent focus reduction method that the three culture-adapted HRV isolates were clearly different antigenically from ovine rotavirus (NCDV) and, further, that a noticeable difference in antigenicity also existed among the HRV isolates.
从含有人类轮状病毒（HRV）的少量腹泻粪便开始，我们使用MA - 104细胞的滚瓶培养技术成功地繁殖了该病毒。此外，我们成功地使HRV适应了静置培养，并开发了针对细胞培养适应病毒的空斑测定法。三种适应细胞培养的病毒分离株KU、YO和44在由0.6%纯化琼脂、3微克乙酰胰蛋白酶/毫升和50微克DEAE - 葡聚糖/毫升组成的覆盖培养基下产生了空斑（直径约0.5 - 1.0毫米）。随后的空斑纯化导致形成清晰、更大的空斑。使用荧光灶减少法进行的交叉中和试验结果表明，三种适应细胞培养的HRV分离株在抗原性上与绵羊轮状病毒（NCDV）明显不同，而且，HRV分离株之间在抗原性上也存在显著差异。
