Spence R P, Pullin J S, Moore N F
Arch Virol. 1986;90(1-2):53-62. doi: 10.1007/BF01314144.
Mill Door/79 virus, an orbivirus of the Kemerovo serogroup, Great Island Complex, was shown to induce in infected cells 10 segments of double-stranded RNA with a total molecular weight of 11.46 X 10(6) daltons. Using methyl mercuric hydroxide as a denaturing agent the double-stranded RNA was translated in a cell-free system producing 11 polypeptides, 10 of which co-migrated with those produced in Mill Door/79 virus infected Vero cells. The segments were separated and individually translated in a cell-free system allowing their coding assignments to be made. These assignments were confirmed by partial proteolysis.
米尔多尔/79病毒是克麦罗沃血清群大岛复合体的一种环状病毒，已证明它能在受感染细胞中诱导产生10个双链RNA片段，其总分子量为11.46×10⁶道尔顿。使用氢氧化甲基汞作为变性剂，双链RNA在无细胞系统中进行翻译，产生11种多肽，其中10种与米尔多尔/79病毒感染的非洲绿猴肾细胞（Vero细胞）中产生的多肽迁移情况相同。这些片段被分离出来，并在无细胞系统中单独进行翻译，从而确定它们的编码任务。这些任务通过部分蛋白酶解得到了证实。
