Spence R P, Eley S M, Nuttall P A, Pullin J S, Moore N F
J Virol. 1985 Feb;53(2):705-7. doi: 10.1128/JVI.53.2.705-707.1985.
The replication and polypeptide synthesis of orbivirus isolate Mill Door/79, a member of the Kemerovo serogroup, were studied. In Vero cells cell-associated virus exceeded cell-free virus by about 2 log10 PFU/ml. Attempts to purify the virus resulted in the demonstration of five polypeptides. Thirteen virus-induced polypeptides and 10 segments of double-stranded RNA were identified in infected cells. Partial proteolysis demonstrated homology between some polypeptides.
研究了凯米罗沃血清群成员鄂毕病毒分离株米尔多尔/79的复制和多肽合成。在Vero细胞中,与细胞相关的病毒比游离病毒高出约2 log10 PFU/ml。纯化该病毒的尝试导致了5种多肽的鉴定。在感染细胞中鉴定出13种病毒诱导的多肽和10段双链RNA。部分蛋白酶解显示一些多肽之间存在同源性。