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米尔门/79病毒的复制与多肽合成，米尔门/79是一种从苏格兰海鸟聚居地的蜱虫中分离出的环状病毒。

Replication and polypeptide synthesis of Mill Door/79, an orbivirus isolated from ticks from a seabird colony in Scotland.

作者信息

Spence R P, Eley S M, Nuttall P A, Pullin J S, Moore N F

出版信息

J Virol. 1985 Feb;53(2):705-7. doi: 10.1128/JVI.53.2.705-707.1985.

DOI:10.1128/JVI.53.2.705-707.1985
PMID:3968722
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC254691/
Abstract

The replication and polypeptide synthesis of orbivirus isolate Mill Door/79, a member of the Kemerovo serogroup, were studied. In Vero cells cell-associated virus exceeded cell-free virus by about 2 log10 PFU/ml. Attempts to purify the virus resulted in the demonstration of five polypeptides. Thirteen virus-induced polypeptides and 10 segments of double-stranded RNA were identified in infected cells. Partial proteolysis demonstrated homology between some polypeptides.

摘要

研究了凯米罗沃血清群成员鄂毕病毒分离株米尔多尔/79的复制和多肽合成。在Vero细胞中，与细胞相关的病毒比游离病毒高出约2 log10 PFU/ml。纯化该病毒的尝试导致了5种多肽的鉴定。在感染细胞中鉴定出13种病毒诱导的多肽和10段双链RNA。部分蛋白酶解显示一些多肽之间存在同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4613/254691/dea722074bd3/jvirol00125-0372-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4613/254691/79ed2f5bfc06/jvirol00125-0371-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4613/254691/cd2ca55bd35d/jvirol00125-0372-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4613/254691/dea722074bd3/jvirol00125-0372-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4613/254691/79ed2f5bfc06/jvirol00125-0371-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4613/254691/cd2ca55bd35d/jvirol00125-0372-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4613/254691/dea722074bd3/jvirol00125-0372-b.jpg

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Replication and polypeptide synthesis of Mill Door/79, an orbivirus isolated from ticks from a seabird colony in Scotland.米尔门/79病毒的复制与多肽合成，米尔门/79是一种从苏格兰海鸟聚居地的蜱虫中分离出的环状病毒。
J Virol. 1985 Feb;53(2):705-7. doi: 10.1128/JVI.53.2.705-707.1985.
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Proteins expressed by Mill Door/79 virus, a Kemerovo serogroup orbivirus transmitted by the ticks Ixodes uriae.米尔门/79病毒（克麦罗沃血清群环状病毒，由海鸟蜱传播）表达的蛋白质。
Arch Virol. 1986;90(1-2):53-62. doi: 10.1007/BF01314144.

本文引用的文献

1
Orbiviruses and bunyaviruses from a seabird colony in Scotland.来自苏格兰一个海鸟聚居地的环状病毒和布尼亚病毒。
J Gen Virol. 1981 Nov;57(Pt 1):127-37. doi: 10.1099/0022-1317-57-1-127.
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In vivo and in vitro synthesis of the proteins expressed by the RNA of black beetle virus.
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On the evolution of orbiviruses.论环状病毒的进化
Intervirology. 1983;20(2-3):169-80. doi: 10.1159/000149388.
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
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Bull World Health Organ. 1969;40(1):113-21.
7
Structure of the bluetongue virus capsid.蓝舌病病毒衣壳的结构。
J Virol. 1972 Oct;10(4):783-94. doi: 10.1128/JVI.10.4.783-794.1972.
8
Isolation of viral double-stranded RNAs using a LiCl fractionation procedure.使用氯化锂分级分离法分离病毒双链RNA。
Prep Biochem. 1978;8(1):1-17. doi: 10.1080/00327487808068215.