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米尔门/79病毒的复制与多肽合成,米尔门/79是一种从苏格兰海鸟聚居地的蜱虫中分离出的环状病毒。

Replication and polypeptide synthesis of Mill Door/79, an orbivirus isolated from ticks from a seabird colony in Scotland.

作者信息

Spence R P, Eley S M, Nuttall P A, Pullin J S, Moore N F

出版信息

J Virol. 1985 Feb;53(2):705-7. doi: 10.1128/JVI.53.2.705-707.1985.

Abstract

The replication and polypeptide synthesis of orbivirus isolate Mill Door/79, a member of the Kemerovo serogroup, were studied. In Vero cells cell-associated virus exceeded cell-free virus by about 2 log10 PFU/ml. Attempts to purify the virus resulted in the demonstration of five polypeptides. Thirteen virus-induced polypeptides and 10 segments of double-stranded RNA were identified in infected cells. Partial proteolysis demonstrated homology between some polypeptides.

摘要

研究了凯米罗沃血清群成员鄂毕病毒分离株米尔多尔/79的复制和多肽合成。在Vero细胞中,与细胞相关的病毒比游离病毒高出约2 log10 PFU/ml。纯化该病毒的尝试导致了5种多肽的鉴定。在感染细胞中鉴定出13种病毒诱导的多肽和10段双链RNA。部分蛋白酶解显示一些多肽之间存在同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4613/254691/79ed2f5bfc06/jvirol00125-0371-a.jpg

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