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面包小麦(L.)某些籽粒性状的数量性状基因座定位

QTL mapping for some grain traits in bread wheat ( L.).

作者信息

Kumari Supriya, Jaiswal Vandana, Mishra Vinod Kumar, Paliwal Rajneesh, Balyan Harindra Singh, Gupta Pushpendra Kumar

机构信息

1Molecular Biology Laboratory, Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut, U.P. India.

2School of Life Science, Jawaharlal Nehru University, New Delhi, India.

出版信息

Physiol Mol Biol Plants. 2018 Sep;24(5):909-920. doi: 10.1007/s12298-018-0552-1. Epub 2018 Jun 1.

Abstract

Grain traits are important agronomic attributes with the market value as well as milling yield of bread wheat. In the present study, quantitative trait loci (QTL) regulating grain traits in wheat were identified. Data for grain area size (GAS), grain width (GWid), factor form density (FFD), grain length-width ratio (GLWR), thousand grain weight (TGW), grain perimeter length (GPL) and grain length (GL) were recorded on a recombinant inbred line derived from the cross of NW1014 × HUW468 at Meerut and Varanasi locations. A linkage map of 55 simple sequence repeat markers for 8 wheat chromosomes was used for QTL analysis by Composite interval mapping. Eighteen QTLs distributed on 8 chromosomes were identified for seven grain traits. Of these, five QTLs for GLWR were found on chromosomes 1A, 6A, 2B, and 7B, three QTLs for GPL were located on chromosomes 4A, 5A and 7B and three QTLs for GAS were mapped on 5D and 7D. Two QTLs were identified on chromosomes 4A and 5A for GL and two QTLs for GWid were identified on chromosomes 7D and 6A. Similarly, two QTLs for FFD were found on chromosomes 1A and 5D. A solitary QTL for TGW was identified on chromosome 2B. For several traits, QTLs were also co-localized on chromosomes 2B, 4A, 5A, 6A, 5D, 7B and 7D. The QTLs detected in the present study may be validated for specific crosses and then used for marker-assisted selection to improve grain quality in bread wheat.

摘要

籽粒性状是面包小麦重要的农艺性状,具有市场价值和制粉产量。在本研究中,鉴定了调控小麦籽粒性状的数量性状位点(QTL)。在密拉特和瓦拉纳西两地,对由NW1014×HUW468杂交产生的重组自交系记录了籽粒面积大小(GAS)、籽粒宽度(GWid)、因子形态密度(FFD)、籽粒长宽比(GLWR)、千粒重(TGW)、籽粒周长(GPL)和籽粒长度(GL)的数据。利用包含8条小麦染色体上55个简单序列重复标记的连锁图谱,通过复合区间作图法进行QTL分析。针对7个籽粒性状,在8条染色体上鉴定出18个QTL。其中,在1A、6A、2B和7B染色体上发现了5个控制GLWR的QTL,在4A、5A和7B染色体上定位到3个控制GPL的QTL,在5D和7D染色体上定位到3个控制GAS的QTL。在4A和5A染色体上鉴定出2个控制GL的QTL,在7D和6A染色体上鉴定出2个控制GWid的QTL。同样,在1A和5D染色体上发现了2个控制FFD的QTL。在2B染色体上鉴定出1个单独的控制TGW的QTL。对于几个性状,QTL也共定位在2B、4A、5A、6A、5D、7B和7D染色体上。本研究中检测到的QTL可针对特定杂交组合进行验证,然后用于标记辅助选择,以改善面包小麦的籽粒品质。

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