氨基糖苷类共轭金纳米颗粒对烧伤患者分离菌株的抗菌活性
Antibacterial Activity of Gold Nanoparticles Conjugated by Aminoglycosides Against Isolates from Burn Patients.
作者信息
Rad Mehdi Rostami, Kazemian Hossein, Yazdani Fateme, Monfared Mohammad Reza Zand, Rahdar Hoseinali, Javadi Ali, Kodori Mansoor
机构信息
Department of Medical microbiology, School of Medicine, Tehran University of Medical Science, Tehran, Iran.
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
出版信息
Recent Pat Antiinfect Drug Discov. 2018;13(3):256-264. doi: 10.2174/1574891X13666180828115543.
BACKGROUND AND OBJECTIVE
Today, resistance to multiple classes of antibiotics, and notably to the β-lactam and aminoglycosides in A. baumannii is becoming a great problem and it necessitates to make a new approach to combat with multidrug-resistant (MDR), extensive drug-resistance (XDR) or Pandrug-resistant (PDR) isolates. In this case, a new strategy and ways should be designed and introduced against such infections. Therefore the aim of the present study was the evaluation of antibacterial activity of nanoconjugate gentamicin and amikacin with gold against clinical isolates of A. baumannii that were collected from burn wound infection. There are some patents of gold nanoparticles that are conjugated with antibiotics (WO2017161296A1, US20090181101A1).
METHODS
Eighteen A. baumannii were collected from burn wound infections. For confirmation and detection of aminoglycoside-resistant genes, PCR was carried out. Gold nanoparticles and nanoconjugates were prepared according to the protocol. For the evaluation of the nanoconjugate, Dynamic light cattering, Transmission electron microscopy and FTIR Analysis were carried out. Then, the antibacterial activity of nanoconjugates was conducted by using micro broth dilution method.
RESULT
Prevalence of aminoglycoside-resistant genes was aacC1, aphA6, aadA1, aadB genes 55.5%, 22.2%, 38.8% and 22.2% respectively. Synthesis of bare nanoconjugates resulted in nanoparticle in a size of 10 nm. Amikacin bound to Gnps showed excellent antibacterial activity (94.5%) and just one isolate showed intermediate resistance. Also, gentamicin bound to Gnps had a good antimicrobial effect (50%) in contrast to gentamicin alone.
CONCLUSION
Our study showed that a combination of amikacin and gentamicin with Gnps has a significant antibacterial efficiency against clinical isolates of A. baumannii. Gnps can be used as extraordinary molecular carriers for targeting, and delivery of the antibiotic molecules to the specific infection.
背景与目的
如今，鲍曼不动杆菌对多种抗生素产生耐药性，尤其是对β-内酰胺类和氨基糖苷类抗生素的耐药性正成为一个重大问题，因此有必要采用新方法来对抗多重耐药（MDR）、广泛耐药（XDR）或全耐药（PDR）菌株。在这种情况下，应设计并引入针对此类感染的新策略和方法。因此，本研究的目的是评估纳米共轭物庆大霉素和阿米卡星与金结合物对从烧伤创面感染中分离出的鲍曼不动杆菌临床菌株的抗菌活性。有一些抗生素与金纳米颗粒结合的专利（WO2017161296A1、US20090181101A1）。
方法
从烧伤创面感染中收集了18株鲍曼不动杆菌。通过PCR进行氨基糖苷类耐药基因的确认和检测。根据方案制备金纳米颗粒和纳米共轭物。为了评估纳米共轭物，进行了动态光散射、透射电子显微镜和傅里叶变换红外光谱分析。然后，采用微量肉汤稀释法进行纳米共轭物的抗菌活性检测。
结果
氨基糖苷类耐药基因aacC1、aphA6、aadA1、aadB的流行率分别为55.5%、22.2%、38.8%和22.2%。裸纳米共轭物的合成产生了尺寸为10nm的纳米颗粒。与金纳米颗粒结合的阿米卡星显示出优异的抗菌活性（94.5%），只有一株菌株表现出中度耐药。此外，与单独的庆大霉素相比，与金纳米颗粒结合的庆大霉素具有良好的抗菌效果（50%）。
结论
我们的研究表明阿米卡星和庆大霉素与金纳米颗粒的组合对鲍曼不动杆菌临床分离株具有显著的抗菌效果。金纳米颗粒可作为特殊的分子载体，用于将抗生素分子靶向并递送至特定感染部位。
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