Trifan Adriana, Aprotosoaie Ana Clara, Cioancă Oana, Hăcianu Monica, Jităreanu Alexandra, Gille Elvira, Miron Anca
Rev Med Chir Soc Med Nat Iasi. 2016 Jul-Sep;120(3):732-6.
Essential oils have significant antioxidant activity, being used frequently as preservatives in the food and cosmetic industries. Aim: To assess the in vitro antioxidant activity of essential oil from Carum carvi L. (caraway) cultivated in northeastern Romania. Materials and Methods: The essential oil was isolated by hydrodistillation from dried caraway fruits. The chemical composition was investigated by gas chromatography and gas chromatography–mass spectrometry analyses. Antioxidant activity was evaluated by three different in vitro antioxidant assays (DPPH∙ and ABTS•+ scavenging and reducing power assays). Butylhydroxyanisole was used as reference standard. Results and Discussion: Carvone was the major compound of essential oil of caraway fruits (48.53%), followed by limonene (44.42%). Evaluation of the antioxidant activity (DPPH∙and ABTS•+ scavenging activity and reducing power) revealed significant effects, with IC50 values of 46.51 ± 1.61 µg/mL, 5.34 ± 0.07 µg/mL and 7.64 ± 0.22 µg/mL, respectively, as compared to those of the reference standard, butylhydroxyanisole (6.09 ± 0.27 µg/mL, 1.49 ± 0.00 µg/mL and 3.39 ± 0.07 µg/mL, respectively). Conclusions: Essential oil of Carum carvi cultivated in northeastern Romania belongs to carvone chemotype. Due to its high antioxidant activity it might be a potential alternative to conventional preservatives in the food, cosmetic and pharmaceutical industries.
香精油具有显著的抗氧化活性，在食品和化妆品工业中经常用作防腐剂。目的：评估罗马尼亚东北部种植的葛缕子（Carum carvi L.）香精油的体外抗氧化活性。材料与方法：通过水蒸馏法从干燥的葛缕子果实中分离出香精油。采用气相色谱和气相色谱 - 质谱联用分析方法研究其化学成分。通过三种不同的体外抗氧化试验（DPPH∙和ABTS•+清除及还原能力试验）评估抗氧化活性。丁基羟基茴香醚用作参考标准。结果与讨论：香芹酮是葛缕子果实香精油的主要成分（48.53%），其次是柠檬烯（44.42%）。抗氧化活性评估（DPPH∙和ABTS•+清除活性及还原能力）显示出显著效果，其IC50值分别为46.51 ± 1.61 µg/mL、5.34 ± 0.07 µg/mL和7.64 ± 0.22 µg/mL，与参考标准丁基羟基茴香醚（分别为6.09 ± 0.27 µg/mL、1.49 ± 0.00 µg/mL和3.39 ± 0.07 µg/mL）相比。结论：罗马尼亚东北部种植的葛缕子香精油属于香芹酮化学型。由于其高抗氧化活性，它可能成为食品、化妆品和制药工业中传统防腐剂的潜在替代品。
