Srivastava B I, Wong-Staal F, Getchell J P
Cancer Res. 1986 Sep;46(9):4756-8.
Antibodies reactive against human T-cell leukemia virus I (HTLV-I) were detected by indirect immunofluorescence assay using MT-2 as target cells, enzyme linked immunosorbent assay screen and competition assay, and Western blot analysis in three sera (one collected in 1979) from a captive gorilla which developed diffuse histiocytic lymphoma in 1983. The sera from four other healthy gorillas housed separately were HTLV-I antibody negative. All sera were negative for HTLV-III antibodies by enzyme linked immunosorbent assay. Southern blot analysis of DNA from lymphoma tissue after digestion with BamHI and using complete HTLV-I genome probe gave one 10-kilobase fragment and a characteristic 1.05-kilobase internal fragment detected in all known HTLV-I isolates. These results indicate that the gorilla was infected with HTLV-I or a closely related simian virus several years before the development of lymphoma.
利用MT - 2作为靶细胞，通过间接免疫荧光测定、酶联免疫吸附测定筛选和竞争测定以及蛋白质印迹分析，在一只圈养大猩猩的三份血清（一份于1979年采集）中检测到了抗人类T细胞白血病病毒I（HTLV - I）的抗体，这只大猩猩于1983年患上弥漫性组织细胞淋巴瘤。另外四只单独饲养的健康大猩猩的血清HTLV - I抗体呈阴性。通过酶联免疫吸附测定，所有血清的HTLV - III抗体均为阴性。用BamHI消化淋巴瘤组织的DNA，并使用完整的HTLV - I基因组探针进行Southern印迹分析，得到了一个10千碱基的片段以及在所有已知HTLV - I分离株中都能检测到的一个特征性1.05千碱基内部片段。这些结果表明，这只大猩猩在患淋巴瘤的数年前就感染了HTLV - I或一种密切相关的猿猴病毒。
