Sato E, Takehara Y, Sasaki J, Matsuno T, Utsumi K
Cell Struct Funct. 1986 Jun;11(2):125-34. doi: 10.1247/csf.11.125.
Polymorphonuclear leukocytes undergo a series of morphological and biochemical changes in response to various chemical stimuli. Transmembrane potential change is an early event that follows stimulation and membrane depolarization may act as a trigger for superoxide generation. To determine if there is a correlation between membrane depolarization and superoxide generation, we investigated the effects of different membrane modulators on stimulus-dependent depolarization. The membrane modulators mepacrine, chlorpromazine and cepharanthine inhibited the superoxide generation produced by chemotactic peptide, FMLP, and/or digitonin in neutrophils. Inhibitory profiles of the activation parameters, however, demonstrate that membrane depolarization is not associated with superoxide generation: FMLP-induced depolarization was inhibited by the modulators tested and was accompanied by the suppression of superoxide generation, but the depolarization produced by digitonin was stimulated somewhat by these drugs. Our results indicate that receptor-mediated membrane depolarization is not a necessary event for the activation of superoxide generation by digitonin.
多形核白细胞会因各种化学刺激而经历一系列形态和生化变化。跨膜电位变化是刺激后的早期事件，膜去极化可能作为超氧化物生成的触发因素。为了确定膜去极化与超氧化物生成之间是否存在相关性，我们研究了不同膜调节剂对刺激依赖性去极化的影响。膜调节剂米帕林、氯丙嗪和千金藤素抑制了趋化肽、FMLP和/或洋地黄皂苷在中性粒细胞中产生的超氧化物生成。然而，激活参数的抑制曲线表明，膜去极化与超氧化物生成无关：FMLP诱导的去极化被测试的调节剂抑制，并伴随着超氧化物生成的抑制，但洋地黄皂苷产生的去极化在某种程度上被这些药物刺激。我们的结果表明，受体介导的膜去极化对于洋地黄皂苷激活超氧化物生成不是必需的事件。
