Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
College of Water Resources and Environment, Hebei Geo University, Shijiazhuang, 050031, China.
Sci Rep. 2018 Aug 28;8(1):12930. doi: 10.1038/s41598-018-30532-4.
This work studied the microbial community in partial nitritation and complete nitrification processes, which were applied to treat the low Carbon Nitrogen ratio wastewater. The phospholipid fatty acid and quantitative PCR analysis showed that the sludge circulating ratio of 75% resulted in a good microbial growth and a higher abundance of ammonia oxidizing bacteria relative to the nitrite oxidizing bacteria. The Betaproteobacteria were observed to compose the most abundant sludge bacterial groups in the two processes, based on phylogenetic analysis. The phylogenetic analysis of both 16S rRNA and amoA gene indicated that the Nitrosomonas sp. were the dominant ammonia oxidizing bacteria in the partial nitritation process. The relative abundance of nitrite oxidizing bacteria, such as Nitrobacter sp. and Nitrospira sp., were significantly lower in the partial nitritation system over the complete nitrification system. The abundance of Planctomycetes was higher in the partial nitritation process, indicating the anammox reaction occurred in the partial nitritation system. These results suggested the nitrite accumulation rate of circulating ratios 75% was the highest, with an average of 92%,and a possibility to treat the low Carbon Nitrogen ratio wastewater using the partial nitritation/anammox process.
本研究采用部分亚硝化和完全硝化工艺处理低碳氮比废水，考察了微生物群落。通过磷脂脂肪酸和定量 PCR 分析表明，与亚硝酸盐氧化菌相比，污泥循环比为 75%时有利于微生物的生长和氨氧化菌的丰度增加。基于系统发育分析，发现 Betaproteobacteria 是两种工艺中最丰富的污泥细菌群。16S rRNA 和 amoA 基因的系统发育分析表明，在部分亚硝化过程中，Nitrosomonas sp. 是优势氨氧化菌。亚硝化系统中亚硝酸盐氧化菌（如 Nitrobacter sp. 和 Nitrospira sp.）的相对丰度明显低于完全硝化系统。部分亚硝化过程中 Planctomycetes 的丰度较高，表明部分亚硝化系统中发生了厌氧氨氧化反应。这些结果表明，循环比为 75%时亚硝酸盐积累率最高，平均为 92%，有可能采用部分亚硝化/厌氧氨氧化工艺处理低碳氮比废水。
