El-Maraghy Nermine, Ghaly Mona S, Dessouki Omar, Nasef Samah Ismail, Metwally Lobna
Department of Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Department of Rheumatology and Rehabilitation, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Arch Med Sci. 2018 Aug;14(5):1033-1040. doi: 10.5114/aoms.2016.63597. Epub 2016 Nov 15.
T regulatory cells (Treg) play an important role in the maintenance of immune cell homeostasis, as it has been reported that CD4+CD25+ T cells suppress the auto-reactive responses in autoimmune diseases such as systemic lupus erythematosus (SLE). The clinical significance of the recently identified population of CD4+CD25-Foxp3+ T cells and whether they are associated with particular organ involvement is still not clear. So, the aim of our study was to evaluate the presence of CD4+CD25-Foxp3+ cells in SLE patients in comparison to healthy controls and to determine whether their frequency is associated with disease activity and particular clinical manifestations in these SLE patients.
The frequency of CD4+CD25-Foxp3+ T cells was analyzed in 56 female SLE patients and 30 healthy female control subjects, using flow cytometry (FACS). CD4+CD25-Foxp3+ T cells were correlated with clinical and laboratory data and the SLE Disease Activity Index (SLEDAI).
The level of CD4+CD25-Foxp3+ T cells was significantly increased in SLE patients (15.57 ±4.32%) as compared with the control group (2.46 ±0.65%). A significant correlation was observed for the percentage of CD4+CD25-Foxp3+ T cells with clinical disease activity scores and disease duration ( = 0.6, < 0.001; = 0.3, = 0.02 respectively). It was also positively correlated with renal impairment and hematological involvement.
Systemic lupus erythematosus patients exhibited an altered level of their CD4+Foxp3+ T cells with increased levels of CD4+CD25-Foxp3+ cells.
调节性T细胞（Treg）在维持免疫细胞稳态中发挥重要作用，据报道，CD4+CD25+ T细胞可抑制自身免疫性疾病如系统性红斑狼疮（SLE）中的自身反应性应答。最近发现的CD4+CD-25Foxp3+ T细胞群体的临床意义以及它们是否与特定器官受累相关仍不清楚。因此，我们研究的目的是评估SLE患者与健康对照相比CD4+CD25-Foxp3+细胞的存在情况，并确定这些SLE患者中其频率是否与疾病活动及特定临床表现相关。
使用流式细胞术（FACS）分析了56例女性SLE患者和30例健康女性对照受试者中CD4+CD25-Foxp3+ T细胞的频率。将CD4+CD25-Foxp3+ T细胞与临床和实验室数据以及SLE疾病活动指数（SLEDAI）进行关联分析。
与对照组（2.46±0.65%）相比，SLE患者中CD4+CD25-Foxp3+ T细胞水平显著升高（15.57±4.32%）。观察到CD4+CD25-Foxp3+ T细胞百分比与临床疾病活动评分和疾病持续时间存在显著相关性（分别为r = 0.6，P < 0.001；r = 0.3，P = 0.02）。它还与肾功能损害和血液系统受累呈正相关。
系统性红斑狼疮患者表现出其CD4+Foxp3+ T细胞水平改变，CD4+CD25-Foxp3+细胞水平升高。
