Yegorenkova Irina V, Fomina Alla A, Tregubova Kristina V, Konnova Svetlana A, Ignatov Vladimir V
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov, 410049, Russian Federation.
Yuri Gagarin State Technical University of Saratov, 77 Ulitsa Politekhnicheskaya, Saratov, 410054, Russian Federation.
Arch Microbiol. 2018 Dec;200(10):1471-1480. doi: 10.1007/s00203-018-1564-5. Epub 2018 Aug 28.
Bacterial polysaccharides are promising stimulants of protective functions in humans and animals. We investigated the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism. We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α)] by human mononuclear cells. The exopolysaccharide promoted the phagocytosis of bacterial cells, activated metabolic processes in human and animal leukocytes, and moderately affected the production of TNF-α and IL-1β. The exopolysaccharides produced on media with glucose and sucrose differed in their effect on the immune cells, possibly owing to their different compositions, structures, and properties. The results validly indicate that the exopolysaccharide of P. polymyxa CCM 1465 promotes nonspecific immunity. Therefore, it can find application as a biologically active immunomodulatory substance.
细菌多糖有望成为人和动物保护功能的刺激物。我们研究了多粘类芽孢杆菌CCM 1465的胞外多糖在大生物体中诱导非特异性抗性因子的能力。我们在体外检测了用不同碳源生产的胞外多糖对小鼠巨噬细胞吞噬活性、活性氧和酶（酸性磷酸酶和髓过氧化物酶）生成、小鼠脾细胞增殖以及人单核细胞促炎细胞因子[白细胞介素-1（IL-1）和肿瘤坏死因子α（TNF-α）]合成的影响。该胞外多糖促进了细菌细胞的吞噬作用，激活了人和动物白细胞中的代谢过程，并适度影响了TNF-α和IL-1β的产生。在含有葡萄糖和蔗糖的培养基上产生的胞外多糖对免疫细胞的影响不同，这可能是由于它们不同的组成、结构和性质。结果有效地表明，多粘类芽孢杆菌CCM 1465的胞外多糖促进非特异性免疫。因此，它可以作为一种生物活性免疫调节物质得到应用。
