从多粘类芽孢杆菌 PYQ1 中提取的胞外多糖的分离、鉴定及抗紫外线辐射的细胞保护作用。
Isolation, characterization and cytoprotective effects against UV radiation of exopolysaccharide produced from Paenibacillus polymyxa PYQ1.
机构信息
College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou 310058, Zhejiang, China.
HeZe (High-tech Zone) BioUnique Biotechnology Co. Ltd, Heze 274000, Shandong, China.
出版信息
J Biosci Bioeng. 2020 Sep;130(3):283-289. doi: 10.1016/j.jbiosc.2020.05.001. Epub 2020 Jun 4.
A novel exopolysaccharide (EPS) from Paenibacillus polymyxa PYQ1 was extracted, well purified and characterized. This EPS was homogeneous glucomannan-type polysaccharide with the average molecular weight of 4.38 × 10 Da. Structural characterization indicated that the monosaccharides of EPS were pyranoses connected by β-glycosidic linkages. Furthermore, our results showed the protective benefits of EPS against UVC induced cytotoxicity in HaCaT cells through scavenging excessive reactive oxygen species, mitigating the decrease of mitochondrial membrane potential, improving catalase activity and maintaining membrane integrity. Taken together, this study qualified EPS from P. polymyxa PYQ1 was a promising natural polymer which worth further investigation as a skin-care agent.
从多粘类芽孢杆菌 PYQ1 中提取、纯化并表征了一种新型胞外多糖 (EPS)。该 EPS 是均一的葡甘露聚糖型多糖，平均分子量为 4.38×10^5 Da。结构表征表明 EPS 的单糖通过β-糖苷键连接形成吡喃糖。此外，我们的研究结果表明，EPS 通过清除过量的活性氧、减轻线粒体膜电位下降、提高过氧化氢酶活性和维持膜完整性，对 UVC 诱导的 HaCaT 细胞的细胞毒性具有保护作用。综上所述，本研究表明多粘类芽孢杆菌 PYQ1 产生的 EPS 是一种很有前途的天然聚合物，作为一种护肤品，值得进一步研究。
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