Harada Gakuro, Onoue Shiori, Inoue Chisato, Hanada Sakae, Katakura Yoshinori
Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka, 812-8581, Japan.
Cytotechnology. 2018 Dec;70(6):1707-1712. doi: 10.1007/s10616-018-0246-0. Epub 2018 Aug 28.
Delphinidin is an anthocyanidin commonly found in various fruits and vegetables. Delphinidin has been known to possess many functions, such as an antioxidant, and anti-inflammatory, anti-cancer and anti-muscular atrophy agent. In this study, we attempted to evaluate the effects of delphinidin on lipid accumulation in hepatocytes. The results showed that palmitic acid (PA)-induced cellular senescence in HepG2 cells and reduced the expression of SMARCD1, which is known to regulate senescence-associated lipid accumulation in hepatocytes. However, delphinidin-3-glucoside (D3 g) suppressed PA-induced senescence and reversed the expression of SMARCD1 to the level of untreated HepG2 cells. Consequently, D3 g inhibited PA-induced lipid accumulation through the restoration of the expression of SMARCD1 and fatty acid oxidation genes. Taken together, our results suggest that D3 g suppresses the lipid accumulation induced by hepatocyte senescence.
飞燕草素是一种常见于各种水果和蔬菜中的花青素。已知飞燕草素具有多种功能，如抗氧化、抗炎、抗癌和抗肌肉萎缩作用。在本研究中，我们试图评估飞燕草素对肝细胞脂质积累的影响。结果表明，棕榈酸（PA）诱导HepG2细胞衰老，并降低了SMARCD1的表达，已知SMARCD1可调节肝细胞衰老相关的脂质积累。然而，飞燕草素-3-葡萄糖苷（D3 g）抑制了PA诱导的衰老，并将SMARCD1的表达恢复到未处理的HepG2细胞水平。因此，D3 g通过恢复SMARCD1和脂肪酸氧化基因的表达来抑制PA诱导的脂质积累。综上所述，我们的结果表明D3 g抑制肝细胞衰老诱导的脂质积累。
