Delphinidin-3--glucoside, an active compound of calyces, inhibits oxidative stress and inflammation in rabbits with atherosclerosis.

CONTEXT

Delphinidin-3--glucoside (DP) is a bioactive compound of L. (Malvaceae) (Roselle) calyces and exerts endothelial protection and lipid-lowering activities, which provided a basis for the prevention and treatment of cardiovascular diseases.

OBJECTIVES

To investigate the therapeutic effects of DP against atherosclerosis.

MATERIALS AND METHODS

A rabbit model of atherosclerosis (AS) was established by 12 weeks of a high-fat diet (HFD). The rabbits were divided into five groups: control, AS, simvastatin (4 mg/kg), and two DP groups (10 and 20 mg/kg). After treatment with DP or simvastatin by oral gavage for 12 weeks, the lipid profiles were measured. Histopathological assessment of the aorta was performed by H&E staining. Oxidative stress and inflammation-related markers were analyzed by ELISA kit and real-time RT-PCR.

RESULTS

DP (20 mg/kg) decreased serum TG (2.36 ± 0.66 4.33 ± 0.27 mmol/L for the AS group), TC, LDL-C, and HDL-C (all  < 0.05). DP (20 mg/kg) also reduced lipid levels in the liver and aorta. DP (20 mg/kg) down-regulated the mRNA levels of IL-6, VCAM-1, and NF-κB and up-regulated the mRNA levels of GSH-PX and SOD1.

CONCLUSIONS

This study proved that DP alleviated the HFD-induced oxidative stress and inflammation in atherosclerosis rabbits. These results provided the scientific basis for developing novel therapies.