1 CNRS UMR 7365, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine, Biopôle de l'Université de Lorraine, Vandoeuvre-Lès-Nancy, France.
2 CHRU de Nancy-Service Odontologie, Vandœuvre-lès-Nancy, France.
Tissue Eng Part B Rev. 2019 Feb;25(1):78-88. doi: 10.1089/ten.TEB.2018.0168. Epub 2018 Oct 9.
Mesenchymal stem cells (MSC) have a lot of potential in regenerative medicine, and MSC-based therapies are currently explored in numerous research fields. Among these cells, deciduous or permanent dental pulp-MSC represent a promising option in tissue engineering. This expectation is based on their capacity to self-renew, to repair various damaged tissues and organs due to their multipotency, as well as their ability to modulate immune system. They present other advantages such as the harvesting by a simple, painless, and noninvasive procedure and the absence of ethical considerations. The role played by these cells in the reparative process is mainly attributed to paracrine mechanisms mediated by their secreted factors, namely the secretome. The secreted factors can be found in the cell culture medium, called conditioned medium (CM). Moreover, CM presents many advantages compared with cells such as possible use in allogeneic therapies. This minireview aims at investigating the therapeutic use of dental pulp MSC-derived CM to develop cell-free therapies. The analysis of the available literature illustrates its massive panel of potential applications: mainly reduction of inflammation, promotion of angiogenesis and neurogenesis, reduction of stroke or ischemia, and organ regeneration. Furthermore, studies often highlight its superiority over the other sources of CM derived from other stem cells for the same applications. Dental pulp MSC-derived CM is an attractive, noninvasive, and acellular tool for therapeutic approaches in regenerative medicine. This promising novel approach should be further explored for clinical applications.
间充质干细胞（MSC）在再生医学中有很大的潜力，基于 MSC 的疗法目前正在众多研究领域中探索。在这些细胞中，乳牙或恒牙牙髓-MSC 是组织工程中很有前途的选择。这种期望基于它们的自我更新能力、由于其多能性而修复各种受损组织和器官的能力，以及调节免疫系统的能力。它们还具有其他优点，例如可以通过简单、无痛、非侵入性的程序进行采集，并且不存在伦理问题。这些细胞在修复过程中发挥作用主要归因于其分泌因子介导的旁分泌机制，即分泌组。分泌因子可以在细胞培养物中找到，称为条件培养基（CM）。此外，与细胞相比，CM 具有许多优势，例如可能用于同种异体疗法。这篇综述旨在研究牙髓 MSC 衍生的 CM 在开发无细胞疗法中的治疗用途。对现有文献的分析说明了其广泛的潜在应用：主要是减轻炎症、促进血管生成和神经发生、减少中风或缺血，以及器官再生。此外，研究经常强调其在用于相同应用的其他干细胞来源的 CM 中的优越性。牙髓 MSC 衍生的 CM 是一种有吸引力的、非侵入性的、无细胞的工具，可用于再生医学中的治疗方法。这种有前途的新方法应该进一步探索用于临床应用。
