Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
Department of Biomedical Imaging, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
J Tissue Eng Regen Med. 2019 Apr;13(4):569-586. doi: 10.1002/term.2806. Epub 2019 Mar 20.
Recent studies suggest that the main driving force behind the therapeutic activity observed in mesenchymal stem cells (MSCs) are the paracrine factors secreted by these cells. These biomolecules also trigger antiapoptotic events to prevent further degeneration of the diseased organ through paracrine signalling mechanisms. In comparison with the normal physiological conditions, an increased paracrine gradient is observed within the peripheral system of diseased organs that enhances the migration of tissue-specific MSCs towards the site of infection or injury to promote healing. Thus, upon administration of conditioned media derived from mesenchymal stem cell cultures (MSC-CM) could contribute in maintaining the increased paracrine factor gradient between the diseased organ and the stem cell niche in order to speed up the process of recovery. Based on the principle of the paracrine signalling mechanism, MSC-CM, also referred as the secretome of the MSCs, is a rich source of the paracrine factors and are being studied extensively for a wide range of regenerative therapies such as myocardial infarction, stroke, bone regeneration, hair growth, and wound healing. This article highlights the current technological applications and advances of MSC-CM with the aim to appraise its future potential as a regenerative therapeutic agent.
最近的研究表明，间充质干细胞（MSCs）治疗活性的主要驱动力是这些细胞分泌的旁分泌因子。这些生物分子还通过旁分泌信号机制触发抗细胞凋亡事件，以防止患病器官进一步退化。与正常生理条件相比，在患病器官的外周系统中观察到旁分泌梯度增加，这增强了组织特异性 MSC 向感染或损伤部位的迁移，以促进愈合。因此，施用源自间充质干细胞培养物的条件培养基（MSC-CM）可能有助于维持患病器官和干细胞龛之间增加的旁分泌因子梯度，以加速恢复过程。基于旁分泌信号机制的原理，MSC-CM 也称为 MSC 的分泌组，是旁分泌因子的丰富来源，并被广泛研究用于各种再生疗法，如心肌梗死、中风、骨再生、毛发生长和伤口愈合。本文重点介绍了 MSC-CM 的当前技术应用和进展，旨在评估其作为再生治疗剂的未来潜力。
