Eppell Steven J, Tong Weidong, McMasters James, Soenjaya Yohannes, Barbu Anca M, Ko Alvin, Baskin Jonathan Z
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA.
Department of Otolaryngology-Head & Neck Surgery, Case Western Reserve University, Cleveland, OH 44106, USA.
Materials (Basel). 2018 Aug 29;11(9):1556. doi: 10.3390/ma11091556.
Material is reviewed that consists of reconstituted collagen fibril gel mineralized in a manner that produces biomimetically sized nanoapatites intimately associated with the fibrils. This gel is formed into usable shapes with a modulus and strength that allow it to be surgically press fitted into bony defects. The design paradigm for the material is that the nanoapatites will dissolve into soluble Ca as the collagen is degraded into RGD-containing peptide fragments due to osteoclastic action. This is intended to signal to the osteoclasts to continue removing the material in a biomimetic fashion similar to bony remodeling. Preliminary experiments in a subcutaneous rat model show that the material is biocompatible with respect to inflammatory and immunogenic responses, and that it supports cellular invasion. Preliminary experiments in a critical-sized mandibular defect in rats show that the material is resorbable and functions well as a bone morphogenetic 2 (BMP-2) carrier. We have produced a range of mechanical and biological responses by varying mechanical and chemical processing of the material.
所审查的材料是由重组胶原纤维凝胶矿化而成，其矿化方式能产生与纤维紧密相关的仿生尺寸纳米磷灰石。这种凝胶被制成具有一定模量和强度的可用形状，使其能够通过手术压配合到骨缺损处。该材料的设计理念是，随着胶原因破骨细胞作用降解为含RGD的肽片段，纳米磷灰石将溶解为可溶性钙。这旨在向破骨细胞发出信号，使其以类似于骨重塑的仿生方式继续清除材料。在大鼠皮下模型中的初步实验表明，该材料在炎症和免疫原性反应方面具有生物相容性，并且支持细胞侵入。在大鼠临界大小的下颌骨缺损中的初步实验表明，该材料是可吸收的，并且作为骨形态发生蛋白2（BMP - 2）载体功能良好。我们通过改变材料的机械和化学加工方式产生了一系列机械和生物学反应。
