长链非编码 RNA LAIR 的过表达增加了水稻的粒产量并调节了邻近基因簇的表达。
Overexpressing lncRNA LAIR increases grain yield and regulates neighbouring gene cluster expression in rice.
机构信息
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, 200438, China.
Research Center for Ecological Science and Technology, Fudan Zhangjiang Institute, Shanghai, 201203, China.
出版信息
Nat Commun. 2018 Aug 29;9(1):3516. doi: 10.1038/s41467-018-05829-7.
Long noncoding RNAs (lncRNAs) are essential regulators of gene expression in eukaryotes. Despite increasing knowledge on the function of lncRNAs, little is known about their effects on crop yield. Here, we identify a lncRNA transcribed from the antisense strand of neighbouring gene LRK (leucine-rich repeat receptor kinase) cluster named LAIR (LRK Antisense Intergenic RNA). LAIR overexpression increases rice grain yield and upregulates the expression of several LRK genes. Additionally, chromatin immunoprecipitation assay results indicate H3K4me3 and H4K16ac are significantly enriched at the activated LRK1 genomic region. LAIR binds histone modification proteins OsMOF and OsWDR5 in rice cells, which are enriched in LRK1 gene region. Moreover, LAIR is demonstrated to bind 5' and 3' untranslated regions of LRK1 gene. Overall, this study reveals the role of lncRNA LAIR in regulating rice grain yield and lncRNAs may be useful targets for crop breeding.
长链非编码 RNA（lncRNA）是真核生物基因表达的重要调控因子。尽管人们对 lncRNA 的功能有了越来越多的了解，但对它们对作物产量的影响却知之甚少。在这里，我们鉴定了一个从邻近基因 LRK（富含亮氨酸重复受体激酶）簇的反义链转录的 lncRNA，命名为 LAIR（LRK 反义基因间 RNA）。LAIR 的过表达增加了水稻的粒产量，并上调了几个 LRK 基因的表达。此外，染色质免疫沉淀实验结果表明，H3K4me3 和 H4K16ac 在激活的 LRK1 基因组区域显著富集。LAIR 在水稻细胞中与组蛋白修饰蛋白 OsMOF 和 OsWDR5 结合，而这两种蛋白在 LRK1 基因区域富集。此外，已经证明 LAIR 可以结合 LRK1 基因的 5'和 3'非翻译区。总的来说，这项研究揭示了 lncRNA LAIR 在调节水稻粒产量中的作用，lncRNA 可能是作物育种的有用靶点。
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