State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China.
Genes (Basel). 2020 Feb 26;11(3):243. doi: 10.3390/genes11030243.
Rice seed is a pivotal reproductive organ that directly determines yield and quality. Long non-coding RNAs (lncRNAs) have been recognized as key regulators in plant development, but the roles of lncRNAs in rice seed development remain unclear. In this study, we performed a paired-end RNA sequencing in samples of rice pistils and seeds at three and seven days after pollination (DAP) respectively. A total of 540 lncRNAs were obtained, among which 482 lncRNAs had significantly different expression patterns during seed development. Results from semi-qPCR conducted on 15 randomly selected differentially expressed lncRNAs suggested high reliability of the transcriptomic data. RNA interference of , which is predominantly transcribed in developing seeds, significantly reduced grain length and thousand-grain weight. These results expanded the dataset of lncRNA in rice and enhanced our understanding of the biological functions of lncRNAs in rice seed development.
水稻种子是一种关键的生殖器官，直接决定着产量和品质。长链非编码 RNA（lncRNA）已被认为是植物发育的关键调节因子，但 lncRNA 在水稻种子发育中的作用尚不清楚。在这项研究中，我们分别在授粉后 3 天和 7 天的水稻雌蕊和种子样本中进行了配对末端 RNA 测序。共获得 540 个 lncRNA，其中 482 个在种子发育过程中表现出明显不同的表达模式。对随机选择的 15 个差异表达 lncRNA 进行的半 qPCR 结果表明转录组数据具有很高的可靠性。在发育中的种子中主要转录的 的 RNA 干扰显著降低了粒长和千粒重。这些结果扩展了水稻 lncRNA 的数据集，并增强了我们对 lncRNA 在水稻种子发育中的生物学功能的理解。
