Mohammadpou Zinat, Amiri Fatemehsadat, Saboor-Yaraghi Ali Akbar, Koohdani Fariba, Norouzzadeh Marjan, Sharifi Loghman, SeyyedSalehi MonirehSadat, Ebrahimi Amirpasha, Mahmoudi Maryam
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
Liver Transplantation Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
Med J Islam Repub Iran. 2018 Feb 10;32:10. doi: 10.14196/mjiri.32.10. eCollection 2018.
Resveratrol (RSV) provides several important biological functions in wide variety of cells. In this study, we investigated the molecular mechanisms underlying anti-inflammatory effect of RSV on HepG2 cells by assessing the gene expression of RelA and c-Jun- subunits of NF-κB and AP-1 transcription factors. HepG2 cells were settled in a serum- free medium with high concentrations of glucose (30 mM) and insulin (1 µM) overnight and were then incubated with RSV (5, 10, and 20 µM) for 24 and 48 hours. Real time quantitative polymerase chain reaction (qRT-PCR) was used to determine RelA and c-Jun expression. RSV diminished hyperglycemia/hyperinsulinemia stimulated expression of c-Jun dose- dependently after 24 and 48 hours (p<0.05). In addition, RelA gene expression was decreased dose-dependently in all RSV doses after 48-hour incubation (p<0.05). Our results indicated that RSV may reduce NF-κB and AP-1 activity via RelA and c-Jun gene regulation. The findings of the present study demonstrated that RSV may be considered as a preventative and therapeutic agent for antagonizing inflammation in Hepatocellular carcinoma (HCC).
白藜芦醇（RSV）在多种细胞中发挥着多种重要的生物学功能。在本研究中，我们通过评估核因子-κB（NF-κB）的RelA亚基和活化蛋白-1（AP-1）转录因子的c-Jun亚基的基因表达，来研究RSV对HepG2细胞抗炎作用的分子机制。将HepG2细胞置于含有高浓度葡萄糖（30 mM）和胰岛素（1 µM）的无血清培养基中过夜，然后用RSV（5、10和20 µM）孵育24小时和48小时。采用实时定量聚合酶链反应（qRT-PCR）测定RelA和c-Jun的表达。RSV在24小时和48小时后剂量依赖性地降低了高血糖/高胰岛素血症刺激的c-Jun表达（p<0.05）。此外，孵育48小时后，所有RSV剂量下RelA基因表达均呈剂量依赖性降低（p<0.05）。我们的结果表明，RSV可能通过RelA和c-Jun基因调控降低NF-κB和AP-1的活性。本研究结果表明，RSV可被视为一种预防和治疗肝细胞癌（HCC）炎症的药物。
